Application
Fig1: Western blot analysis of AKT1/2/3 on MCF-7 cell lysates using anti-AKT1/2/3 antibody at 1/1,000 dilution.Application
Fig2: ICC staining AKT1/2/3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining AKT1/2/3 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining AKT1/2/3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of A549 cells with AKT1/2/3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | AKT1/2/3 [ST48-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT antibody AKT1 antibody AKT1 kinase antibody AKT1m antibody AKT2 antibody AKT2 kinase antibody Akt3 antibody AKT3_HUMAN antibody CAKT antibody CWS6 antibody DKFZp434N0250 antibody HIHGHH antibody kinase Akt1 antibody MGC99656 antibody MPPH antibody Murine thymoma viral (v-akt) homolog 2 antibody PKB ALPHA antibody PKB antibody PKB beta antibody PKB gamma antibody PKB-GAMMA antibody PKB/Akt antibody PKBALPHA antibody PKBB antibody PKBBETA antibody PKBG antibody PKBGAMMA antibody PRKBA antibody PRKBB antibody PRKBG antibody Protein kinase Akt 2 antibody Protein kinase Akt-3 antibody Protein kinase B alpha antibody Protein kinase B antibody Protein kinase B beta antibody Protein kinase B gamma antibody Proto oncogene c Akt antibody RAC ALPHA antibody RAC alpha serine/threonine protein kinase antibody RAC antibody RAC BETA antibody RAC beta serine/threonine protein kinase antibody RAC PK alpha antibody RAC PK beta antibody rac protein kinase alpha antibody rac protein kinase beta antibody RAC-gamma antibody RAC-gamma serine/threonine-protein kinase antibody RAC-PK-gamma antibody RACALPHA antibody RACalpha serine/threonine kinase antibody RACBETA antibody RACgamma antibody RACgamma serine/threonine protein kinase antibody RACPKgamma antibody serine threonine protein kinase antibody STK-2 antibody STK2 antibody thymoma viral proto oncogene 1 antibody thymoma viral proto oncogene antibody V akt murine thymoma viral oncogene homolog 1 antibody V akt murine thymoma viral oncogene homolog 2 antibody V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody V akt murine thymoma viral oncogene homolog 3 antibody vakt murine thymoma viral oncogene homolog 1 antibody vakt murine thymoma viral oncogene homolog 2 antibody vakt murine thymoma viral oncogene homolog 3 antibody |
| Product description | The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AKT antibody
AKT1 antibody
AKT1 kinase antibody
AKT1m antibody
AKT2 antibody
AKT2 kinase antibody
Akt3 antibody
AKT3_HUMAN antibody
CAKT antibody
CWS6 antibody
DKFZp434N0250 antibody
HIHGHH antibody
kinase Akt1 antibody
MGC99656 antibody
MPPH antibody
Murine thymoma viral (v-akt) homolog 2 antibody
PKB ALPHA antibody
PKB antibody
PKB beta antibody
PKB gamma antibody
PKB-GAMMA antibody
PKB/Akt antibody
PKBALPHA antibody
PKBB antibody
PKBBETA antibody
PKBG antibody
PKBGAMMA antibody
PRKBA antibody
PRKBB antibody
PRKBG antibody
Protein kinase Akt 2 antibody
Protein kinase Akt-3 antibody
Protein kinase B alpha antibody
Protein kinase B antibody
Protein kinase B beta antibody
Protein kinase B gamma antibody
Proto oncogene c Akt antibody
RAC ALPHA antibody
RAC alpha serine/threonine protein kinase antibody
RAC antibody
RAC BETA antibody
RAC beta serine/threonine protein kinase antibody
RAC PK alpha antibody
RAC PK beta antibody
rac protein kinase alpha antibody
rac protein kinase beta antibody
RAC-gamma antibody
RAC-gamma serine/threonine-protein kinase antibody
RAC-PK-gamma antibody
RACALPHA antibody
RACalpha serine/threonine kinase antibody
RACBETA antibody
RACgamma antibody
RACgamma serine/threonine protein kinase antibody
RACPKgamma antibody
serine threonine protein kinase antibody
STK-2 antibody
STK2 antibody
thymoma viral proto oncogene 1 antibody
thymoma viral proto oncogene antibody
V akt murine thymoma viral oncogene homolog 1 antibody
V akt murine thymoma viral oncogene homolog 2 antibody
V akt murine thymoma viral oncogene homolog 3 (protein kinase B gamma) antibody V akt murine thymoma viral oncogene homolog 3 antibody vakt murine thymoma viral oncogene homolog 1 antibody vakt murine thymoma viral oncogene homolog 2 antibody vakt murine thymoma viral oncogene homolog 3 antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Nucleus, Cytoplasm, Membrane. |
Application
Fig1: Western blot analysis of AKT1/2/3 on MCF-7 cell lysates using anti-AKT1/2/3 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining AKT1/2/3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining AKT1/2/3 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining AKT1/2/3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of A549 cells with AKT1/2/3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, MCF-7, CRC, SH-SY-5Y, mouse heart tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
