Application
Fig1: Western blot analysis of CD4 on human thymus tissue lysates using anti-CD4 antibody at 1/1,000 dilution.Application
Fig2: ICC staining CD4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD4 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD4 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD4 antibody. Counter stained with hematoxylin.| Product Name | CD4 [ST0488] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 4 antibody CD4 (L3T4) antibody CD4 antibody CD4 antigen (p55) antibody CD4 antigen antibody CD4 molecule antibody CD4 receptor antibody CD4+ Lymphocyte deficiency, included antibody CD4_HUMAN antibody CD4mut antibody L3T4 antibody Leu3 antibody Ly-4 antibody Lymphocyte antigen CD4 antibody MGC165891 antibody OTTHUMP00000238897 antibody p55 antibody T cell antigen T4 antibody T cell antigen T4/LEU3 antibody T cell differentiation antigen L3T4 antibody T cell OKT4 deficiency, included antibody T cell surface antigen T4/Leu 3 antibody T cell surface antigen T4/Leu3 antibody T cell surface glycoprotein CD4 antibody T-cell surface antigen T4/Leu-3 antibody T-cell surface glycoprotein CD4 antibody W3/25 antibody W3/25 antigen antibody |
| Product description | The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8; T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells. CD4 (also designated T4 and Leu 3), is a membrane glycoprotein that contains four extracellular immunoglobin-like domains. The TCR in association with CD4 can bind class II MHC molecules presented by the antigen-presenting cells. The CD4 protein functions by increasing the avidity of the interaction between the TCR and an antigen-class II MHC complex. An additional role of CD4 is to function as a receptor for HIV. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD 4 antibody
CD4 (L3T4) antibody
CD4 antibody
CD4 antigen (p55) antibody
CD4 antigen antibody
CD4 molecule antibody
CD4 receptor antibody
CD4+ Lymphocyte deficiency included antibody CD4_HUMAN antibody CD4mut antibody L3T4 antibody Leu3 antibody Ly-4 antibody Lymphocyte antigen CD4 antibody MGC165891 antibody OTTHUMP00000238897 antibody p55 antibody T cell antigen T4 antibody T cell antigen T4/LEU3 antibody T cell differentiation antigen L3T4 antibody T cell OKT4 deficiency included antibody T cell surface antigen T4/Leu 3 antibody T cell surface antigen T4/Leu3 antibody T cell surface glycoprotein CD4 antibody T-cell surface antigen T4/Leu-3 antibody T-cell surface glycoprotein CD4 antibody W3/25 antibody W3/25 antigen antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P01730 |
|---|
Application
Fig1: Western blot analysis of CD4 on human thymus tissue lysates using anti-CD4 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining CD4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD4 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD4 antibody. Counter stained with hematoxylin.| Positive Control | A549, CRC, human tonsil tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
