Application
Fig1: Western blot analysis of PIM1 on different lysates using anti-PIM1 antibody at 1/1,000 dilution. Positive control: Lane 1: BT-20 Lane 2: HepG2 Lane 3: SW480Application
Fig2: ICC staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.| Product Name | PIM1 [ST0513] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Oncogene PIM 1 antibody Oncogene PIM1 antibody PIM 1 antibody pim 1 kinase 44 kDa isoform antibody Pim 1 kinase antibody pim 1 oncogene (proviral integration site 1) antibody Pim 1 oncogene antibody PIM antibody PIM1 antibody pim1 kinase 44 kDa isoform antibody PIM1_HUMAN antibody Pim2 antibody PIM3 antibody Proto oncogene serine/threonine protein kinase Pim 1 antibody Proto-oncogene serine/threonine-protein kinase Pim-1 antibody Proviral integration site 1 antibody Proviral integration site 2 antibody |
| Product description | Pim-1 is a serine/threonine kinase that cooperates with c-Myc in lymphoid cell transformation. The expression of pim-1 increases during the progression from early to late G1, remaining high at the G1/S boundary and G2 phases of the cell cycle. Pim-1 is regulated at both the transcriptional and translational level, and it has been shown to be induced by IL-2 stimulation. Pim-1 also plays a role in T cell differentiation, and it has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Oncogene PIM 1 antibody Oncogene PIM1 antibody PIM 1 antibody pim 1 kinase 44 kDa isoform antibody Pim 1 kinase antibody pim 1 oncogene (proviral integration site 1) antibody Pim 1 oncogene antibody PIM antibody PIM1 antibody pim1 kinase 44 kDa isoform antibody PIM1_HUMAN antibody Pim2 antibody PIM3 antibody Proto oncogene serine/threonine protein kinase Pim 1 antibody Proto-oncogene serine/threonine-protein kinase Pim-1 antibody Proviral integration site 1 antibody Proviral integration site 2 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell membrane. |
Application
Fig1: Western blot analysis of PIM1 on different lysates using anti-PIM1 antibody at 1/1,000 dilution. Positive control: Lane 1: BT-20 Lane 2: HepG2 Lane 3: SW480
Application
Fig2: ICC staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.| Positive Control | BT-20, SW480, HepG2, human breast carcinoma tissue, human tonsil tissue, human gastric carcinoma tissue, human colon cancer tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
