Application
Fig1: Western blot analysis of Cyclophilin B on different lysates using anti-Cyclophilin B antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: NIH/3T3| Product Name | Cyclophilin B [ST05-22] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AA408962 antibody AA553318 antibody AI844835 antibody Cphn 2 antibody Cphn2 antibody Cyclophilin B antibody Cyclophilin like protein antibody CyP 20b antibody CYP S1 antibody CYP-S1 antibody CYPB antibody EC 5.2.1.8 antibody MGC14109 antibody MGC2224 antibody OI9 antibody peptidyl prolyl cis trans isomerase B antibody Peptidyl prolyl cis trans isomerase B precursor antibody Peptidyl-prolyl cis-trans isomerase B antibody peptidylprolyl isomerase B (cyclophilin B) antibody Peptidylprolyl isomerase B antibody PPIase antibody PPIase B antibody Ppib antibody PPIB_HUMAN antibody Rotamase antibody Rotamase B antibody S cyclophilin antibody S-cyclophilin antibody SCYLP antibody |
| Product description | The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the Cyclosporin A and is encoded by a single unique gene conserved from yeast to humans. CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication. Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:1,000-1:5,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AA408962 antibody AA553318 antibody AI844835 antibody Cphn 2 antibody Cphn2 antibody Cyclophilin B antibody Cyclophilin like protein antibody CyP 20b antibody CYP S1 antibody CYP-S1 antibody CYPB antibody EC 5.2.1.8 antibody MGC14109 antibody MGC2224 antibody OI9 antibody peptidyl prolyl cis trans isomerase B antibody Peptidyl prolyl cis trans isomerase B precursor antibody Peptidyl-prolyl cis-trans isomerase B antibody peptidylprolyl isomerase B (cyclophilin B) antibody Peptidylprolyl isomerase B antibody PPIase antibody PPIase B antibody Ppib antibody PPIB_HUMAN antibody Rotamase antibody Rotamase B antibody S cyclophilin antibody S-cyclophilin antibody SCYLP antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Endoplasmic reticulum lumen, Melanosome. |
Application
Fig1: Western blot analysis of Cyclophilin B on different lysates using anti-Cyclophilin B antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: NIH/3T3| Positive Control | NIH/3T3, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
