Application
Fig1: Western blot analysis of HIF-1 beta on human liver lysates using anti-HIF-1 beta antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HIF-1 beta antibody. Counter stained with hematoxylin.| Product Name | HIF-1 beta [ST05-25] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | arnT antibody ARNT protein antibody ARNT_HUMAN antibody Aryl hydrocarbon receptor nuclear translocator antibody bHLHe2 antibody Class E basic helix-loop-helix protein 2 antibody Dioxin receptor antibody Dioxin receptor nuclear translocator antibody Drnt antibody HIF 1 beta antibody HIF 1beta antibody HIF-1-beta antibody HIF1-beta antibody HIF1B antibody HIF1beta antibody Hypoxia Inducible Factor 1 antibody Hypoxia inducible factor 1 beta antibody Hypoxia-inducible factor 1-beta antibody nuclear translocator antibody Tango antibody |
| Product description | AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. Arnt 2 (aryl hydrocarbon receptor nuclear translocator 2), also known as Hif-2b or bHLHe1, is a 712 amino acid nuclear protein that is exclusively expressed in adult brain and kidney. Containing a basic helix-loop-helix (bHLH) domain, a PAC (PAS-associated C-terminal) domain and two PAS (PER-ARNT-SIM) domains, Arnt 2 specifically recognizes the xenobiotic response element (XRE). |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:1,000-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | arnT antibody ARNT protein antibody ARNT_HUMAN antibody Aryl hydrocarbon receptor nuclear translocator antibody bHLHe2 antibody Class E basic helix-loop-helix protein 2 antibody Dioxin receptor antibody Dioxin receptor nuclear translocator antibody Drnt antibody HIF 1 beta antibody HIF 1beta antibody HIF-1-beta antibody HIF1-beta antibody HIF1B antibody HIF1beta antibody Hypoxia Inducible Factor 1 antibody Hypoxia inducible factor 1 beta antibody Hypoxia-inducible factor 1-beta antibody nuclear translocator antibody Tango antibody |
|---|---|
| Molecular Weight(MW) | 87 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P27540 |
|---|
Application
Fig1: Western blot analysis of HIF-1 beta on human liver lysates using anti-HIF-1 beta antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HIF-1 beta antibody. Counter stained with hematoxylin.| Positive Control | Human liver tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
