Application
Fig1: Western blot analysis of Neuropilin-1 on different lysates using anti-Neuropilin-1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Mouse liver Lane 3: Mouse kidney Lane 4: Human liverApplication
Fig2: ICC staining Neuropilin-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Neuropilin-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Neuropilin-1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Hela cells with Neuropilin-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Neuropilin-1 [ST05-30] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A5 protein antibody BDCA4 antibody BLOOD DENDRITIC CELL ANTIGEN 4 antibody CD304 antibody Neuropilin-1 antibody Neuropilin1 antibody NP1 antibody NPN1 antibody NRP 1 antibody NRP antibody NRP1 antibody NRP1_HUMAN antibody transmembrane receptor antibody Vascular endothelial cell growth factor 165 receptor antibody VEGF165R antibody |
| Product description | Neuropilin is a type I transmembrane receptor that has been implicated in aspects of axon growth and guidance and has been shown to act as a high affinity receptor for class III semaphorins and vascular endothelial growth factor (VEGF). A closely related protein, neuropilin-2, shares a common domain structure and signifcant homology with neuropilin and also acts as a receptor for the class III semaphorins and VEGF. Both neuropilins are involved in regulating many physiological pathways including axonal guidance and angiogenesis, however they exhibit differential expression in the adult vasculature. Neuropilin-2 is polysialylated and expressed on the surface of dendritic cells. It is also expressed by venous and lymphatic endothelium. Neuropilin is expressed predominantly by arterial endothelium. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | A5 protein antibody BDCA4 antibody BLOOD DENDRITIC CELL ANTIGEN 4 antibody CD304 antibody Neuropilin-1 antibody Neuropilin1 antibody NP1 antibody NPN1 antibody NRP 1 antibody NRP antibody NRP1 antibody NRP1_HUMAN antibody transmembrane receptor antibody Vascular endothelial cell growth factor 165 receptor antibody VEGF165R antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Cell membrane, Secreted. |
Application
Fig1: Western blot analysis of Neuropilin-1 on different lysates using anti-Neuropilin-1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Mouse liver Lane 3: Mouse kidney Lane 4: Human liver
Application
Fig2: ICC staining Neuropilin-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Neuropilin-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Neuropilin-1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with Neuropilin-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | HUVEC, MCF-7, SHG-44, mouse kidney tissue, human liver tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
