Application
Fig1: Western blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1,000 dilution.Application
Fig2: ICC staining Sodium Potassium ATPase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Sodium Potassium ATPase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Hela cells with Sodium Potassium ATPase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Sodium Potassium ATPase [ST0533] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATPase Na+/K+ transporting alpha antibody adenosinetriphosphatase antibody AT1A1_HUMAN antibody ATP1A1 antibody ATP1A4 antibody ATP1AL2 antibody ATP1B antibody ATP1B1 antibody ATPase Na+/K+ transporting alpha 1 polypeptide antibody ATPase Na+/K+ transporting alpha 4 polypeptide antibody ATPase Na+/K+ transporting beta 1 polypeptide antibody ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody ATPase, Na+/K+ transporting, beta 1 polypeptide antibody Beta 1-subunit of Na(+),K(+)-ATPase antibody Na(+)/K(+) ATPase alpha-1 subunit antibody Na(+)/K(+) ATPase alpha-4 subunit antibody Na+, K+ ATPase alpha subunit antibody Na+/K+ ATPase 1 antibody Na+/K+ ATPase 4 antibody Na+/K+ ATPase, alpha-D polypeptide antibody Na, K-ATPase beta-1 polypeptide antibody Na, K-ATPase, alpha-A catalytic polypeptide antibody Na,K-ATPase catalytic subunit alpha-A protein antibody Na,K-ATPase subunit alpha-C antibody polypeptide-like 2 antibody Sodium pump 1 antibody sodium pump 4 antibody Sodium pump subunit alpha-1 antibody sodium pump subunit alpha-4 antibody sodium pump subunit beta-1 antibody sodium-potassium ATPase catalytic subunit alpha-1 antibody sodium-potassium ATPase catalytic subunit alpha-4 antibody sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody sodium-potassium ATPase, alpha 4 polypeptide antibody sodium-potassium-ATPase, alpha 1 polypeptide antibody Sodium/potassium transporting ATPase alpha 1 chain antibody Sodium/potassium transporting ATPase subunit beta 1 antibody sodium/potassium-dependent ATPase beta-1 subunit antibody sodium/potassium-transporting ATPase alpha-4 chain antibody sodium/potassium-transporting ATPase beta-1 chain antibody Sodium/potassium-transporting ATPase subunit alpha-1 antibody sodium/potassium-transporting ATPase subunit alpha-4 antibody |
| Product description | The ubiquitously expressed sodium/potassium-ATPase (Na;+;/K;+;-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na;+; ions and two K;+; ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na;+;/K;+;-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na;+;/K;+;-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na;+;/K;+;-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na;+;/K;+;-ATPase. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ATPase Na+/K+ transporting alpha antibody
adenosinetriphosphatase antibody AT1A1_HUMAN antibody ATP1A1 antibody ATP1A4 antibody ATP1AL2 antibody ATP1B antibody ATP1B1 antibody ATPase Na+/K+ transporting alpha 1 polypeptide antibody ATPase Na+/K+ transporting alpha 4 polypeptide antibody ATPase Na+/K+ transporting beta 1 polypeptide antibody ATPase Na+/K+ transporting alpha polypeptide-like 2 antibody ATPase Na+/K+ transporting beta 1 polypeptide antibody Beta 1-subunit of Na(+) K(+)-ATPase antibody Na(+)/K(+) ATPase alpha-1 subunit antibody Na(+)/K(+) ATPase alpha-4 subunit antibody Na+ K+ ATPase alpha subunit antibody Na+/K+ ATPase 1 antibody Na+/K+ ATPase 4 antibody Na+/K+ ATPase alpha-D polypeptide antibody Na K-ATPase beta-1 polypeptide antibody Na K-ATPase alpha-A catalytic polypeptide antibody Na K-ATPase catalytic subunit alpha-A protein antibody Na K-ATPase subunit alpha-C antibody polypeptide-like 2 antibody Sodium pump 1 antibody sodium pump 4 antibody Sodium pump subunit alpha-1 antibody sodium pump subunit alpha-4 antibody sodium pump subunit beta-1 antibody sodium-potassium ATPase catalytic subunit alpha-1 antibody sodium-potassium ATPase catalytic subunit alpha-4 antibody sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody sodium-potassium ATPase alpha 4 polypeptide antibody sodium-potassium-ATPase alpha 1 polypeptide antibody Sodium/potassium transporting ATPase alpha 1 chain antibody Sodium/potassium transporting ATPase subunit beta 1 antibody sodium/potassium-dependent ATPase beta-1 subunit antibody sodium/potassium-transporting ATPase alpha-4 chain antibody sodium/potassium-transporting ATPase beta-1 chain antibody Sodium/potassium-transporting ATPase subunit alpha-1 antibody sodium/potassium-transporting ATPase subunit alpha-4 antibody |
|---|---|
| Molecular Weight(MW) | 100 kDa |
| Cellular Localization | Cell membrane, Melanosome. |
Application
Fig1: Western blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Sodium Potassium ATPase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Sodium Potassium ATPase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with Sodium Potassium ATPase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, MCF-7, Hela, HepG2, human liver tissue, mouse liver tissue, mouse kidney tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
