Application
Fig1: ICC staining Histone H2B(mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Histone H2B(mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Histone H2B(mono methyl R79) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.| Product Name | Histone H2B(mono methyl R79) [ST0430] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2B 1A antibody H2B antibody H2B histone family antibody H2B2f antibody H2B2F_HUMAN antibody H2Ba antibody H2Bf antibody HIST2H2BF antibody histone H2B antibody histone H2B type 1 antibody Histone H2B type 2-F antibody MGC131639 antibody |
| Product description | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Immunogen | recombinant protein |
| Modification | mono methyl R79 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | H2B 1A antibody H2B antibody H2B histone family antibody H2B2f antibody H2B2F_HUMAN antibody H2Ba antibody H2Bf antibody HIST2H2BF antibody histone H2B antibody histone H2B type 1 antibody Histone H2B type 2-F antibody MGC131639 antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Nucleus, Chromosome. |
Application
Fig1: ICC staining Histone H2B(mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Histone H2B(mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Histone H2B(mono methyl R79) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.| Positive Control | MCF-7, Hela, HepG2, human tonsil tissue, mouse testis tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
