Application
Fig1: Western blot analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Cytokeratin 16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Cytokeratin 16 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of HepG2 cells with Cytokeratin 16 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Cytokeratin 16 [SC52-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CK 16 antibody CK-16 antibody CK16 antibody Cytokeratin-16 antibody Cytokeratin16 antibody FNEPPK antibody Focal non epidermolytic palmoplantar keratoderma antibody K 16 antibody K16 antibody K1C16_HUMAN antibody K1CP antibody Keratin 1 type I antibody Keratin 16 antibody Keratin antibody Keratin type I cytoskeletal 16 antibody Keratin-16 antibody Keratin16 antibody KRT 16 antibody Krt16 antibody KRT16A antibody NEPPK antibody PC1 antibody type I cytoskeletal 16 antibody |
| Product description | Cytokeratins comprise a diverse group of intermediate filament proteins that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in noninvasive breast carcinomas and normal breast tissue. Mutations in the Cytokeratin 16 gene cause various diseases, including pachyonychia congenita type 1 (PC1), nonepidermolytic palmoplantar keratoderma (NEPPK) and unilateral palmoplantar verrucous nevus (UPVN). |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CK 16 antibody CK-16 antibody CK16 antibody Cytokeratin-16 antibody Cytokeratin16 antibody FNEPPK antibody Focal non epidermolytic palmoplantar keratoderma antibody K 16 antibody K16 antibody K1C16_HUMAN antibody K1CP antibody Keratin 1 type I antibody Keratin 16 antibody Keratin antibody Keratin type I cytoskeletal 16 antibody Keratin-16 antibody Keratin16 antibody KRT 16 antibody Krt16 antibody KRT16A antibody NEPPK antibody PC1 antibody type I cytoskeletal 16 antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoskeleton. |
| SwissProt ID | P08779 |
|---|
Application
Fig1: Western blot analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Cytokeratin 16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cytokeratin 16 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of HepG2 cells with Cytokeratin 16 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, Hela, HepG2, human breast carcinoma tissue, human tonsil tissue, human lung cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
