Application
Fig1: Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1,000 dilution.Application
Fig2: ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | Nanog [SC05-70] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Embryonic stem cell specific homeobox protein (Nanog) antibody ENK antibody FLJ12581 antibody hNanog antibody Homeobox protein NANOG antibody Homeobox transcription factor Nanog antibody homeobox transcription factor Nanog-delta 48 antibody NANOG antibody Nanog homeobox antibody NANOG_HUMAN antibody |
| Product description | Nanog (from “Tir Na Nog,” the mythologic Celtic land of the ever young) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression have been detected by Northern analysis in the undifferentiated NTERA-2 cl.D1 embryonal carcinoma cell line. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Embryonic stem cell specific homeobox protein (Nanog) antibody ENK antibody FLJ12581 antibody hNanog antibody Homeobox protein NANOG antibody Homeobox transcription factor Nanog antibody homeobox transcription factor Nanog-delta 48 antibody NANOG antibody Nanog homeobox antibody NANOG_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9H9S0 |
|---|
Application
Fig1: Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | F9, NCCIT. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
