Application
Fig1: Western blot analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: HUVECApplication
Fig2: ICC staining UBC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining UBC9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. Counter stained with hematoxylin.| Product Name | UBC9 [SC0534] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C358B7.1 antibody p18 antibody SUMO 1 protein ligase antibody SUMO conjugating enzyme UBC9 antibody SUMO-conjugating enzyme UBC9 antibody SUMO-protein ligase antibody SUMO1 protein ligase antibody UBC9 antibody UBC9_HUMAN antibody UBCE9 antibody Ube2i antibody Ubiquitin carrier protein 9 antibody Ubiquitin carrier protein antibody Ubiquitin carrier protein I antibody Ubiquitin conjugating enzyme 9 antibody Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody Ubiquitin conjugating enzyme UbcE2A antibody Ubiquitin like protein SUMO 1 conjugating enzyme antibody Ubiquitin protein ligase E2I antibody Ubiquitin-conjugating enzyme E2 I antibody Ubiquitin-protein ligase I antibody |
| Product description | UBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPChIP |
| WB:1:500- 1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | C358B7.1 antibody
p18 antibody
SUMO 1 protein ligase antibody
SUMO conjugating enzyme UBC9 antibody
SUMO-conjugating enzyme UBC9 antibody
SUMO-protein ligase antibody
SUMO1 protein ligase antibody
UBC9 antibody
UBC9_HUMAN antibody
UBCE9 antibody
Ube2i antibody
Ubiquitin carrier protein 9 antibody
Ubiquitin carrier protein antibody
Ubiquitin carrier protein I antibody
Ubiquitin conjugating enzyme 9 antibody
Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody
Ubiquitin conjugating enzyme E2I (UBC9 homolog yeast) antibody Ubiquitin conjugating enzyme UbcE2A antibody Ubiquitin like protein SUMO 1 conjugating enzyme antibody Ubiquitin protein ligase E2I antibody Ubiquitin-conjugating enzyme E2 I antibody Ubiquitin-protein ligase I antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
Application
Fig1: Western blot analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: HUVEC
Application
Fig2: ICC staining UBC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining UBC9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. Counter stained with hematoxylin.| Positive Control | HUVEC, A431, HepG2, human kidney tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500- 1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
