Application
Fig1: Western blot analysis of COX2 on different lysates using anti-COX2 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: THP-1Application
Fig2: ICC staining COX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining COX2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining COX2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | COX2/Cyclooxygenase 2 [SC56-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COX 2 antibody COX-2 antibody COX2 antibody Cyclooxygenase 2 antibody Cyclooxygenase 2b antibody Cyclooxygenase antibody Cyclooxygenase-2 antibody Cyclooxygenase2 antibody EC 1.14.99.1 antibody fj02a10 antibody Glucocorticoid-regulated inflammatory cyclooxygenase antibody Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody GRIPGHS antibody hCox 2 antibody Macrophage activation-associated marker protein P71/73 antibody OTTHUMP00000033524 antibody PES-2 antibody PGG/HS antibody PGH synthase 2 antibody PGH2_HUMAN antibody PGHS 2 antibody PGHS-2 antibody PGHS2 antibody PHS 2 antibody PHS II antibody PHS2 antibody Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody Prostaglandin endoperoxide synthase 2 antibody Prostaglandin G/H synthase 2 antibody Prostaglandin G/H synthase 2 precursor antibody Prostaglandin G/H synthase and cyclooxygenase antibody Prostaglandin G/H synthase antibody Prostaglandin H2 synthase 2 antibody prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody Prostaglandin-endoperoxide synthase 2 antibody PTGS2 antibody ptgs2a antibody TIS10 antibody TIS10 protein antibody unp1239 antibody wu:fj02a10 antibody |
| Product description | Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutively expressed in most tissues and is thought to serve in general “housekeeping” functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IF |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | COX 2 antibody COX-2 antibody COX2 antibody Cyclooxygenase 2 antibody Cyclooxygenase 2b antibody Cyclooxygenase antibody Cyclooxygenase-2 antibody Cyclooxygenase2 antibody EC 1.14.99.1 antibody fj02a10 antibody Glucocorticoid-regulated inflammatory cyclooxygenase antibody Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody GRIPGHS antibody hCox 2 antibody Macrophage activation-associated marker protein P71/73 antibody OTTHUMP00000033524 antibody PES-2 antibody PGG/HS antibody PGH synthase 2 antibody PGH2_HUMAN antibody PGHS 2 antibody PGHS-2 antibody PGHS2 antibody PHS 2 antibody PHS II antibody PHS2 antibody Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody Prostaglandin endoperoxide synthase 2 antibody Prostaglandin G/H synthase 2 antibody Prostaglandin G/H synthase 2 precursor antibody Prostaglandin G/H synthase and cyclooxygenase antibody Prostaglandin G/H synthase antibody Prostaglandin H2 synthase 2 antibody prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody Prostaglandin-endoperoxide synthase 2 antibody PTGS2 antibody ptgs2a antibody TIS10 antibody TIS10 protein antibody unp1239 antibody wu:fj02a10 antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Microsome membrane, Endoplasmic reticulum membrane. |
Application
Fig1: Western blot analysis of COX2 on different lysates using anti-COX2 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: THP-1
Application
Fig2: ICC staining COX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining COX2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining COX2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | A549, THP-1, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
