WB
Western blot analysis of Aurora B on different lysates using anti-Aurora B antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Aurora B antibody. Counter stained with hematoxylin.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Aurora B with Rabbit anti-Aurora B antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aurora B antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Product Name | Aurora B Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Aurora related kinase-1 (ARK-1, STK15, Aurora2, Aik1) and -2 (ARK-2, STK12, Aurora1) are centrosome-associated serine/ threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. ARK-1 and -2 are expressed in the nucleus and localize to distinct portions of mitotic machinery such as the centrosome, spindle poles (ARK-1), and midbody (ARK-2) during mitosis. ARK-1 and -2 transcripts are present at high levels in human thymus and fetal liver. ARK-1 protein has elevated expression in colon carcinoma lines (HT-29, SNU-C2B, COLO 205, SW480, 837 and 948) and accumulates during metaphase in HeLa cells. ARK-2 protein levels are maximal during both S and G2/M phases, whereas ARK-1 protein is degraded after G2/M via the ubiquitin-proteasome pathway. ARK-2 has a unique genetic loci relative to ARK-1, suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression. |
| Immunogen | Synthetic peptide within Human Aurora B aa 1-50 / 344. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:50-1:200 ICC/IF:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | AIK2 antibody AIM-1 antibody AIM1 antibody ARK-2 antibody ARK2 antibody AurB antibody AURKB antibody AURKB_HUMAN antibody Aurora 1 antibody Aurora and Ipl1 like midbody associated protein 1 antibody Aurora kinase B antibody Aurora related kinase 2 antibody Aurora- and Ipl1-like midbody-associated protein 1 antibody Aurora-B antibody Aurora-related kinase 2 antibody Aurora/IPL1 related kinase 2 antibody Aurora/IPL1-related kinase 2 antibody IPL1 antibody PPP1R48 antibody Protein phosphatase 1 regulatory subunit 48 antibody Serine/theronine kinase 12 antibody Serine/threonine protein kinase 12 antibody Serine/threonine-protein kinase 12 antibody Serine/threonine-protein kinase aurora-B antibody STK-1 antibody STK1 antibody STK12 antibody STK5 antibody |
|---|---|
| Molecular Weight(MW) | 39kDa |
| Cellular Localization | Nucleus, Cytoplasm, Chromosome. |
WB
Western blot analysis of Aurora B on different lysates using anti-Aurora B antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Aurora B antibody. Counter stained with hematoxylin.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Aurora B with Rabbit anti-Aurora B antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aurora B antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Application Notes | WB:1:1000-1:2000 IHC:1:50-1:200 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
