Application
Fig1: ICC staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.| Product Name | Phospho-PKA R2 (S99) [SC54-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cAMP dependent protein kinase regulatory subunit alpha 2 antibody cAMP dependent protein kinase regulatory subunit RII alpha antibody cAMP dependent protein kinase type II alpha regulatory chain antibody cAMP dependent protein kinase type II alpha regulatory subunit antibody cAMP-dependent protein kinase type II-alpha regulatory subunit antibody KAP2 antibody KAP2_HUMAN antibody MGC3606 antibody PKR 2 antibody PKR2 antibody PRKA R2 antibody PRKAR 2 antibody PRKAR2 antibody PRKAR2A antibody Protein kinase A RII alpha subunit antibody Protein kinase cAMP dependent regulatory type II alpha antibody |
| Product description | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex. |
| Immunogen | recombinant protein |
| Modification | p-S99 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRatPig |
| Concentration | 1mg/ml |
| Alternative Names | cAMP dependent protein kinase regulatory subunit alpha 2 antibody cAMP dependent protein kinase regulatory subunit RII alpha antibody cAMP dependent protein kinase type II alpha regulatory chain antibody cAMP dependent protein kinase type II alpha regulatory subunit antibody cAMP-dependent protein kinase type II-alpha regulatory subunit antibody KAP2 antibody KAP2_HUMAN antibody MGC3606 antibody PKR 2 antibody PKR2 antibody PRKA R2 antibody PRKAR 2 antibody PRKAR2 antibody PRKAR2A antibody Protein kinase A RII alpha subunit antibody Protein kinase cAMP dependent regulatory type II alpha antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoplasm, Cell membrane. |
Application
Fig1: ICC staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.| Positive Control | MCF-7, Hela, human spleen tissue, mouse testis tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
