Application
Fig1: Western blot analysis of Estrogen Receptor alpha(phospho S118) on MCF-7 cell lysates using anti- phospho-Estrogen Receptor alpha(S118) antibody at 1/1,000 dilution.Application
Fig2: ICC staining phospho-Estrogen Receptor alpha(S118) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining phospho-Estrogen Receptor alpha(S118) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining phospho-Estrogen Receptor alpha(S118) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho-Estrogen Receptor alpha(S118) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti- phospho-Estrogen Receptor alpha(S118) antibody. Counter stained with hematoxylin.| Product Name | Phospho-Estrogen Receptor alpha(S118) [SC05-87] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686N23123 antibody ER alpha antibody ER antibody ER-alpha antibody Era antibody ESR antibody ESR1 antibody ESR1_HUMAN antibody ESRA antibody Estradiol receptor antibody Estrogen nuclear receptor alpha antibody Estrogen receptor 1 antibody Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody Estrogen receptor alpha antibody Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody Estrogen receptor alpha delta 4 +49 isoform antibody Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody Estrogen receptor alpha E1 E2 1 2 antibody Estrogen receptor alpha E1 N2 E2 1 2 antibody Estrogen receptor antibody ESTRR antibody NR3A1 antibody Nuclear receptor subfamily 3 group A member 1 antibody |
| Product description | Estrogen receptor alpha (ERa, ER, ESR, ESRA, Era, NR3A1, estrogen receptor 1) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. Alternative splicing results in several ERa mRNA transcripts, which differ primarily in their 5' untranslated regions. ERa undergoes phosphorylation in response to estradiol binding. Human ERa is predominately phosphorylated on Ser 118 and to a lesser extent on Ser 104 and Ser 106. In response to activation of the mitogen-activated protein kinase pathway, phosphorylation occurs on Ser 118 and Ser 167. These serine residues are all located within the activation function 1 region of the N-terminal domain of ERa. In contrast, activation of protein kinase A increases the phosphorylation of Ser 236, which is located in the DNA-binding domain. Src kinase-dependent Tyr 537 phosphorylation may enhance estrogen binding to ERa. Mutation of Tyr 537 of the human ERa produces receptors having a range of constitutive activity. |
| Immunogen | recombinant protein |
| Modification | p-S118 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp686N23123 antibody
ER alpha antibody
ER antibody
ER-alpha antibody
Era antibody
ESR antibody
ESR1 antibody
ESR1_HUMAN antibody
ESRA antibody
Estradiol receptor antibody
Estrogen nuclear receptor alpha antibody
Estrogen receptor 1 antibody
Estrogen receptor alpha 3* 4 5 6 7*/822 isoform antibody Estrogen receptor alpha antibody Estrogen receptor alpha delta 3* 4 5 6 7* 8*/941 isoform antibody Estrogen receptor alpha delta 3* 4 5 6 7*/819 2 isoform antibody Estrogen receptor alpha delta 4 +49 isoform antibody Estrogen receptor alpha delta 4* 5 6 7*/654 isoform antibody Estrogen receptor alpha delta 4* 5 6 7 8*/901 isoform antibody Estrogen receptor alpha E1 E2 1 2 antibody Estrogen receptor alpha E1 N2 E2 1 2 antibody Estrogen receptor antibody ESTRR antibody NR3A1 antibody Nuclear receptor subfamily 3 group A member 1 antibody |
|---|---|
| Molecular Weight(MW) | 66 kDa |
| Cellular Localization | Nucleus, Cytoplasm, Cell membrane. |
| SwissProt ID | P03372 |
|---|
Application
Fig1: Western blot analysis of Estrogen Receptor alpha(phospho S118) on MCF-7 cell lysates using anti- phospho-Estrogen Receptor alpha(S118) antibody at 1/1,000 dilution.
Application
Fig2: ICC staining phospho-Estrogen Receptor alpha(S118) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining phospho-Estrogen Receptor alpha(S118) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining phospho-Estrogen Receptor alpha(S118) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho-Estrogen Receptor alpha(S118) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti- phospho-Estrogen Receptor alpha(S118) antibody. Counter stained with hematoxylin.| Positive Control | HUVEC, MCF-7, Hela, human breast carcinoma tissue, mouse placenta tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
