Application
Fig1: Western blot analysis of phospho -TrkB(Y817) on mouse brain lysates using anti- phospho -TrkB(Y817) antibody at 1/1,000 dilution.Application
Fig2: ICC staining phospho -TrkB(Y817) in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining phospho -TrkB(Y817) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.| Product Name | Phospho-TrkB(Y817) [SC0556] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by NTRK2, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3. |
| Immunogen | recombinant protein |
| Modification | p-Y817 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 145 kDa |
|---|---|
| Cellular Localization | Cell membrane, Endosome membrane. |
Application
Fig1: Western blot analysis of phospho -TrkB(Y817) on mouse brain lysates using anti- phospho -TrkB(Y817) antibody at 1/1,000 dilution.
Application
Fig2: ICC staining phospho -TrkB(Y817) in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining phospho -TrkB(Y817) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.| Positive Control | PANC-1, SH-SY-5Y, human kidney tissue, mouse heart tissue, human pancreas tissue, mouse pancreas tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
