Application
Fig1: Western blot analysis of hnRNP K on different lysates using anti-hnRNP K antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: NIH/3T3Application
Fig2: ICC staining hnRNP K in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining hnRNP K in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining hnRNP K in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.| Product Name | hnRNP K [SC60-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CSBP antibody dC stretch binding protein antibody FLJ41122 antibody Heterogeneous nuclear ribonucleoprotein K antibody hnRNP K antibody HNRNPK antibody HNRPK antibody HNRPK_HUMAN antibody Transformation up regulated nuclear protein antibody Transformation up-regulated nuclear protein antibody Transformation upregulated nuclear protein antibody TUNP antibody |
| Product description | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CSBP antibody dC stretch binding protein antibody FLJ41122 antibody Heterogeneous nuclear ribonucleoprotein K antibody hnRNP K antibody HNRNPK antibody HNRPK antibody HNRPK_HUMAN antibody Transformation up regulated nuclear protein antibody Transformation up-regulated nuclear protein antibody Transformation upregulated nuclear protein antibody TUNP antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell projection. |
Application
Fig1: Western blot analysis of hnRNP K on different lysates using anti-hnRNP K antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: NIH/3T3
Application
Fig2: ICC staining hnRNP K in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining hnRNP K in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining hnRNP K in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.| Positive Control | SKOV-3, Jurkat, NIH/3T3, Hela, HepG2, mouse brain tissue, human colon cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
