Application
Fig1: Western blot analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Human skinApplication
Fig2: ICC staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cytokeratin 14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Cytokeratin 14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.| Product Name | Cytokeratin 14 [SC65-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CK 14 antibody CK-14 antibody ck14 antibody Cytokeratin 14 antibody Cytokeratin-14 antibody Cytokeratin14 antibody Dowling Meara antibody EBS3 antibody EBS4 antibody Epidermolysis bullosa simplex antibody K14 antibody K1C14_HUMAN antibody Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody Keratin 14 antibody Keratin antibody Keratin type I cytoskeletal 14 antibody Keratin, type I cytoskeletal 14 antibody Keratin-14 antibody Keratin14 antibody Koebner antibody Krt 14 antibody Krt14 antibody NFJ antibody OTTHUMP00000164624 antibody type I cytoskeletal 14 antibody |
| Product description | arkers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. In Bowen’s disease, the characteristic malignancy of the epidermis exhibits distinct expression patterns of Cytokeratin 14. The gene encoding human Cytokeratin 14 maps to chromosome 17q12-21. Mutations in this gene lead to epidermolysis bullosa simplex, an inheritied skin disorder characterized by skin blistering due to basal keratinocyte fragility. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CK 14 antibody
CK-14 antibody
ck14 antibody
Cytokeratin 14 antibody
Cytokeratin-14 antibody
Cytokeratin14 antibody
Dowling Meara antibody
EBS3 antibody
EBS4 antibody
Epidermolysis bullosa simplex antibody
K14 antibody
K1C14_HUMAN antibody
Keratin 14 (epidermolysis bullosa simplex Dowling-Meara Koebner) antibody Keratin 14 antibody Keratin antibody Keratin type I cytoskeletal 14 antibody Keratin type I cytoskeletal 14 antibody Keratin-14 antibody Keratin14 antibody Koebner antibody Krt 14 antibody Krt14 antibody NFJ antibody OTTHUMP00000164624 antibody type I cytoskeletal 14 antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Human skin
Application
Fig2: ICC staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cytokeratin 14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cytokeratin 14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.| Positive Control | A431, B16-F1, SW480, HepG2, human breast carcinoma tissue, mouse prostate tissue, mouse skin tissue, mouse prostate tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
