Application
Fig1: Western blot analysis of MelanA on different lysates using anti-MelanA antibody at 1/1,000 dilution. Positive control: Lane 1: Melanoma Lane 2: B16-F1Application
Fig2: Flow cytometric analysis of B16-F1 cells with MelanA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seconda antibody| Product Name | MelanA [SC56-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen LB39 AA antibody Antigen LB39-AA antibody Antigen SK29 AA antibody Antigen SK29-AA antibody MAR1_HUMAN antibody MART 1 antibody MART-1 antibody MART1 antibody Melan A antibody Melan A protein antibody Melanoma antigen recognized by T cells 1 antibody Melanoma antigen recognized by T-cells 1 antibody MLAN A antibody MLANA antibody OTTHUMP00000021036 antibody OTTHUMP00000021037 antibody OTTHUMP00000021038 antibody Protein Melan-A antibody |
| Product description | Melanoma-associated antigens recognized by cytotoxic T lymphocytes (CTL) have been grouped into three categories: melanocyte differentiation antigens, cancer/testis-specific antigens and mutated or aberrantly expressed antigens. Many of these antigens consist of peptides that are presented to T cells by HLA molecules; they represent potential targets for cancer immunotherapy. Melan-A (also designated MART-1) is a melanocyte differentiation antigen that is specific to melanomas, melanocyte cell lines and retina. Melan-A peptide is recognized by most HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes in patients with melanoma. Antimelanoma cytotoxic T lymphocytes can be generated with a Melan-A peptide, implicating Melan-A as a potential candidate for antigen-specific immunotherapy in melanoma patients. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Antigen LB39 AA antibody Antigen LB39-AA antibody Antigen SK29 AA antibody Antigen SK29-AA antibody MAR1_HUMAN antibody MART 1 antibody MART-1 antibody MART1 antibody Melan A antibody Melan A protein antibody Melanoma antigen recognized by T cells 1 antibody Melanoma antigen recognized by T-cells 1 antibody MLAN A antibody MLANA antibody OTTHUMP00000021036 antibody OTTHUMP00000021037 antibody OTTHUMP00000021038 antibody Protein Melan-A antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Endoplasmic reticulum membrane, Golgi apparatus, Melanosome. |
| SwissProt ID | Q16655 |
|---|
Application
Fig1: Western blot analysis of MelanA on different lysates using anti-MelanA antibody at 1/1,000 dilution. Positive control: Lane 1: Melanoma Lane 2: B16-F1
Application
Fig2: Flow cytometric analysis of B16-F1 cells with MelanA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seconda antibody| Positive Control | B16-F1, Melanoma. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
