Application
Fig1: Western blot analysis of Phospho-Stat5(Y694) on mouse liver lysates using anti-Phospho-Stat5(Y694) antibody at 1/1,000 dilution.| Product Name | Phospho-Stat5(Y694) [SC05-31] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGF antibody Signal transducer and activator of transcription 5A antibody Signal Transducer and Activator of Transcription 5B antibody STA5A_HUMAN antibody STAT 5A antibody STAT 5B antibody STAT5 antibody STAT5A antibody STAT5B antibody Transcription factor STAT5A antibody Transcription factor STAT5B antibody |
| Product description | Stat5 (Signal Transducers and Activators of Transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5a and Stat5b in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines. IL-2 differentially induces serine phosphorylation of Stat5a and Stat5b on Ser726 and Ser731, respectively. Stat5b is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5a. Both the acid-rich region and the COOH terminus of IL-2Rb can independently mediate IL-2-induced Stat 5a/b serine phosphorylation, suggesting that Stat5a/b serine phosphorylation occurs at a postreceptor level. Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response. |
| Immunogen | recombinant protein |
| Modification | p-Y694 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:1,000-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | MGF antibody Signal transducer and activator of transcription 5A antibody Signal Transducer and Activator of Transcription 5B antibody STA5A_HUMAN antibody STAT 5A antibody STAT 5B antibody STAT5 antibody STAT5A antibody STAT5B antibody Transcription factor STAT5A antibody Transcription factor STAT5B antibody |
|---|---|
| Molecular Weight(MW) | 90 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Phospho-Stat5(Y694) on mouse liver lysates using anti-Phospho-Stat5(Y694) antibody at 1/1,000 dilution.| Positive Control | Mouse liver tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
