Application
Fig1: Western blot analysis of eIF5A on different lysates using anti-eIF5A antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: MCF-7Application
Fig2: ICC staining eIF5A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining eIF5A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-eIF5A antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Jurkat cells with eIF5A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | eIF5A [SC60-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | eIF 4D antibody eIF 5A 1 antibody EIF 5A antibody eIF 5A1 antibody eIF-4D antibody eIF-5A antibody eIF-5A-1 antibody eIF-5A1 antibody eIF4D antibody eif5a antibody EIF5A1 antibody eIF5AI antibody Eukaryotic initiation factor 5A antibody Eukaryotic initiation factor 5A isoform 1 antibody Eukaryotic translation initiation factor 5A 1 antibody Eukaryotic translation initiation factor 5A antibody Eukaryotic translation initiation factor 5A-1 antibody IF5A1_HUMAN antibody MGC104255 antibody MGC99547 antibody Rev binding factor antibody Rev-binding factor antibody uORF A antibody uORF antibody |
| Product description | In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The human eukaryotic translation initiation factor 5A gene, also designated eIF-4D or eIF5A, maps to chromosome 17p13.1 and encodes a 154 amino acid protein that is linked to cellular polyamine homeostasis. eIF5A localizes to the nuclear and cytoplasmic compartments of mammalian cells where it can stimulate ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport and/or protein translation. eIF5A contains a unique spermidine-derived post-translational modification at Lys-50, hypusine, which is necessary for eIF5A's biochemical activity and for cellular proliferative signaling. In addition, eIF5A is a cellular cofactor for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). Inhibition of eIF5A interaction with Rev leads to a block of the viral replication cycle. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | eIF 4D antibody eIF 5A 1 antibody EIF 5A antibody eIF 5A1 antibody eIF-4D antibody eIF-5A antibody eIF-5A-1 antibody eIF-5A1 antibody eIF4D antibody eif5a antibody EIF5A1 antibody eIF5AI antibody Eukaryotic initiation factor 5A antibody Eukaryotic initiation factor 5A isoform 1 antibody Eukaryotic translation initiation factor 5A 1 antibody Eukaryotic translation initiation factor 5A antibody Eukaryotic translation initiation factor 5A-1 antibody IF5A1_HUMAN antibody MGC104255 antibody MGC99547 antibody Rev binding factor antibody Rev-binding factor antibody uORF A antibody uORF antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Endoplasmic reticulum membrane. |
Application
Fig1: Western blot analysis of eIF5A on different lysates using anti-eIF5A antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: MCF-7
Application
Fig2: ICC staining eIF5A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining eIF5A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Jurkat cells with eIF5A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, MCF-7, Hela, HepG2, human kidney tissue, mouse liver tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
