Application
Fig1: Western blot analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution.Application
Fig2: ICC staining PRP19 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PRP19 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining PRP19 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PRP19 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRP19 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PRP19 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Hela cells with PRP19 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | PRP19 [SC06-53] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hPso4 antibody NMP200 antibody Nuclear matrix protein 200 antibody Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody pre mRNA processing factor 19 antibody Pre-mRNA-processing factor 19 antibody PRP19 antibody PRP19/PSO4 homolog antibody PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody PRP19_HUMAN antibody PRPF19 antibody PSO4 antibody psoralen 4 antibody Senescence evasion factor antibody SNEV antibody UBOX4 antibody |
| Product description | The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5' splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | hPso4 antibody NMP200 antibody Nuclear matrix protein 200 antibody Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody pre mRNA processing factor 19 antibody Pre-mRNA-processing factor 19 antibody PRP19 antibody PRP19/PSO4 homolog antibody PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody PRP19_HUMAN antibody PRPF19 antibody PSO4 antibody psoralen 4 antibody Senescence evasion factor antibody SNEV antibody UBOX4 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Nucleus, Cytoplasm, Lipid droplet. |
Application
Fig1: Western blot analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining PRP19 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PRP19 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PRP19 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with PRP19 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, MCF-7, Hela, B16-F1, mouse brain tissue, human lung cancer tissue, mouse kidney tissue, human breast carcinoma tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
