Application
Fig1: Western blot analysis of Myoglobin on human skeletal muscle lysates using anti-Myoglobin antibody at 1/1,000 dilution.Application
Fig2: ICC staining Myoglobin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Myoglobin antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myoglobin antibody. Counter stained with hematoxylin.| Product Name | Myoglobin [SC06-38] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MB antibody MGC13548 antibody MYG_HUMAN antibody Myoglobin antibody PVALB antibody |
| Product description | Myoglobin is a cytosolic oxygen binding protein responsible for the storage and diffusion of oxygen within myocytes. Expression of myoglobin is highest in skeletal and cardiac muscle. Myoglobin is necessary for the maintenance of mitochondrial respiration during heavy and sustained contractile activity, and it is thought to transport oxygen from erythroyctes to mitochondria. The genomic structure of myoglobin appears to be conserved across a broad range of species, and contains a putative polyadenylation signal and a polypyrimidine-rich region. Human myoglobin is specified by a single gene, and it has been identified in human smooth muscle. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | MB antibody MGC13548 antibody MYG_HUMAN antibody Myoglobin antibody PVALB antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Myoglobin on human skeletal muscle lysates using anti-Myoglobin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Myoglobin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Myoglobin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myoglobin antibody. Counter stained with hematoxylin.| Positive Control | C2C12, human skeletal muscle tissue, mouse skeletal muscle tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
