Application
Fig1: Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution. Positive control: Lane 1: THP-1 Lane 2: human heart Lane 3: U937Application
Fig2: ICC staining CD13 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD13 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CD13 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD13 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD13 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD13 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD13 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CD13 antibody. Counter stained with hematoxylin.Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD13 antibody. Counter stained with hematoxylin.| Product Name | CD13 [SC70-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alanyl (membrane) aminopeptidase antibody Alanyl aminopeptidase antibody Aminopeptidase M antibody Aminopeptidase N antibody AMPN_HUMAN antibody ANPEP antibody AP M antibody AP N antibody AP-M antibody AP-N antibody APN antibody CD 13 antibody CD13 antibody CD13 antigen antibody gp150 antibody hAPN antibody LAP 1 antibody LAP1 antibody Microsomal aminopeptidase antibody Myeloid plasma membrane glycoprotein CD13 antibody p150 antibody PEPN antibody |
| Product description | CD13, or aminopeptidase N, is a type II transmembrane glycoprotein that is expressed on most cells of Myeloid origin, including monocytes, basophils, eosinophils, neutrophils and Myeloid leukemias. CD13 is also found on certain epithelial cells, fibroblasts and osteoclasts. CD13 acts as a zinc-binding metalloprotease that plays a role in digestion and may function in the inactivation of some regulatory peptides such as enkephalins. CD13 may play a role in the invasion of cancer cells by enhancing their invasive capacity and metastatic behavior. The activity of CD13 can be inactivated using specific inhibitors that evoke apoptosis of CD13-positive cancer cells. Basic fibroblast growth factor (bFGF) expression upregulates CD13 expression in human melanoma cells by activating both the Myeloid and the epithelial CD13 promoter. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIP |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Alanyl (membrane) aminopeptidase antibody Alanyl aminopeptidase antibody Aminopeptidase M antibody Aminopeptidase N antibody AMPN_HUMAN antibody ANPEP antibody AP M antibody AP N antibody AP-M antibody AP-N antibody APN antibody CD 13 antibody CD13 antibody CD13 antigen antibody gp150 antibody hAPN antibody LAP 1 antibody LAP1 antibody Microsomal aminopeptidase antibody Myeloid plasma membrane glycoprotein CD13 antibody p150 antibody PEPN antibody |
|---|---|
| Molecular Weight(MW) | 150 kDa |
| Cellular Localization | Cell membrane, Cytoplasm. |
Application
Fig1: Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution. Positive control: Lane 1: THP-1 Lane 2: human heart Lane 3: U937
Application
Fig2: ICC staining CD13 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD13 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CD13 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD13 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD13 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD13 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD13 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CD13 antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD13 antibody. Counter stained with hematoxylin.| Positive Control | THP-1, U937, AGS, SHG-44, LO2, human kidney tissue, human tonsil tissue, mouse colon tissue, human liver cancer tissue, mouse brain tissue, human breast tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
