Application
Fig1: Western blot analysis of GART on different lysates using anti-GART antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431Application
Fig2: ICC staining GART in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining GART in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining GART in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GART antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-GART antibody. Counter stained with hematoxylin.| Product Name | GART [SC66-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5'-phosphoribosylglycinamide transformylase antibody AIR synthase antibody AIRS antibody GAR transformylase antibody GARS antibody GART antibody GARTF antibody Glycinamide ribonucleotide synthetase antibody MGC47764 antibody PAIS antibody PGFT antibody Phosphoribosyl-aminoimidazole synthetase antibody Phosphoribosylglycinamide formyltransferase antibody Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase antibody Phosphoribosylglycinamide formyltransferase, EC 2.1.2.29 antibody Phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase antibody Phosphoribosylglycinamide synthetase antibody PRGS antibody PUR2_HUMAN antibody Trifunctional purine biosynthetic protein adenosine 3 antibody |
| Product description | Purines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 5'-phosphoribosylglycinamide transformylase antibody
AIR synthase antibody
AIRS antibody
GAR transformylase antibody
GARS antibody
GART antibody
GARTF antibody
Glycinamide ribonucleotide synthetase antibody
MGC47764 antibody
PAIS antibody
PGFT antibody
Phosphoribosyl-aminoimidazole synthetase antibody
Phosphoribosylglycinamide formyltransferase antibody
Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase antibody
Phosphoribosylglycinamide formyltransferase EC 2.1.2.29 antibody Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase antibody Phosphoribosylglycinamide synthetase antibody PRGS antibody PUR2_HUMAN antibody Trifunctional purine biosynthetic protein adenosine 3 antibody |
|---|---|
| Molecular Weight(MW) | 107 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Membrane. |
Application
Fig1: Western blot analysis of GART on different lysates using anti-GART antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431
Application
Fig2: ICC staining GART in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining GART in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining GART in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GART antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-GART antibody. Counter stained with hematoxylin.| Positive Control | SW480, A431, Hela, HepG2, mouse uterus tissue, human tonsil tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
