Application
Fig1: ICC staining ATG9A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining ATG9A in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATG9A antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATG9A antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATG9A antibody. Counter stained with hematoxylin.| Product Name | ATG9A [SC67-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APG9 autophagy 9-like 1 antibody APG9 like 1 antibody APG9-like 1 antibody APG9L1 antibody ATG9 antibody ATG9 autophagy related 9 homolog A antibody ATG9 autophagy related 9 homolog A (S. cerevisiae) antibody ATG9A antibody ATG9A_HUMAN antibody Autophagy 9-like 1 protein antibody Autophagy related protein 9A antibody Autophagy-related protein 9A antibody mATG9 antibody MGD3208 antibody OTTHUMP00000206046 antibody OTTHUMP00000206048 antibody OTTHUMP00000206049 antibody OTTHUMP00000206062 antibody |
| Product description | Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagy-related gene (Atg) proteins. Atg9a (autophagy-related protein 9A), also known as APG9-like 1, is a 839 amino acid, multi-pass membrane protein that localizes to the pre-autophagosomal structure (PAS). Isolation membranes are suggested to originate from the PAS, enwrapping cytoplasmic components to form a double membrane autophagosome, which then fuses with the vacuole. Ubiquitously expressed in human adult tissues, Atg9a cycles between the Golgi and endosomes and, with the autophagosome-specific marker LC3, plays a critical role in starvation-induced autophagosome formation. Three isoforms of Atg9a exist as a result of alternative splicing events. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIP |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | APG9 autophagy 9-like 1 antibody APG9 like 1 antibody APG9-like 1 antibody APG9L1 antibody ATG9 antibody ATG9 autophagy related 9 homolog A antibody ATG9 autophagy related 9 homolog A (S. cerevisiae) antibody ATG9A antibody ATG9A_HUMAN antibody Autophagy 9-like 1 protein antibody Autophagy related protein 9A antibody Autophagy-related protein 9A antibody mATG9 antibody MGD3208 antibody OTTHUMP00000206046 antibody OTTHUMP00000206048 antibody OTTHUMP00000206049 antibody OTTHUMP00000206062 antibody |
|---|---|
| Molecular Weight(MW) | 94 kDa |
| Cellular Localization | Cytoplasmic vesicle, Golgi apparatus, Late endosome membrane, Endoplasmic reticulum membrane. |
Application
Fig1: ICC staining ATG9A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining ATG9A in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATG9A antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATG9A antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATG9A antibody. Counter stained with hematoxylin.| Positive Control | 293, Hela, rat brain tissue, mouse brain tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
