Application
Fig1: ICC staining MyD88 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining MyD88 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MyD88 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MyD88 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MyD88 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with MyD88 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | MyD88 [SC65-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mutant myeloid differentiation primary response 88 antibody MYD 88 antibody Myd88 antibody MYD88_HUMAN antibody MYD88D antibody Myeloid differentiation marker 88 antibody Myeloid differentiation primary response 88 antibody Myeloid differentiation primary response gene (88) antibody Myeloid differentiation primary response gene 88 antibody Myeloid differentiation primary response gene antibody Myeloid differentiation primary response protein MyD88 antibody OTTHUMP00000161718 antibody OTTHUMP00000208595 antibody OTTHUMP00000209058 antibody OTTHUMP00000209059 antibody OTTHUMP00000209060 antibody |
| Product description | Interleukin-1 (IL-1)-induced activation of the NFκB pathway is mediated through the IL-1 receptor and the subsequent phosphorylation of IL-1 receptor-associated kinase (IRAK). The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NFκB activation and an adjacent Toll/IL-1R homology domain (TIR domain). Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Mutant myeloid differentiation primary response 88 antibody MYD 88 antibody Myd88 antibody MYD88_HUMAN antibody MYD88D antibody Myeloid differentiation marker 88 antibody Myeloid differentiation primary response 88 antibody Myeloid differentiation primary response gene (88) antibody Myeloid differentiation primary response gene 88 antibody Myeloid differentiation primary response gene antibody Myeloid differentiation primary response protein MyD88 antibody OTTHUMP00000161718 antibody OTTHUMP00000208595 antibody OTTHUMP00000209058 antibody OTTHUMP00000209059 antibody OTTHUMP00000209060 antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q99836 |
|---|
Application
Fig1: ICC staining MyD88 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining MyD88 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MyD88 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MyD88 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MyD88 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with MyD88 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | A549, MCF-7, HepG2, human tonsil tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
