Application
Fig1: Western blot analysis of PYK2 on mouse brain lysates using anti-PYK2 antibody at 1/1,000 dilution.Application
Fig2: ICC staining PYK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PYK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PYK2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PYK2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PYK2 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.| Product Name | PYK2 [SC06-15] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CADTK antibody CAK-beta antibody CAKB antibody CAKbeta antibody Calcium regulated non receptor proline rich tyrosine kinase antibody Calcium-dependent tyrosine kinase antibody Cell adhesion kinase beta antibody E430023O05Rik antibody EC 2.7.10.2 antibody FADK 2 antibody FADK2 antibody FAK2 antibody FAK2_HUMAN antibody Focal adhesion kinase 2 antibody MGC124628 antibody PKB antibody Proline-rich tyrosine kinase 2 antibody Protein kinase B antibody Protein Tyrosine Kinase 2 Beta antibody Protein-tyrosine kinase 2-beta antibody PTK antibody PTK2B antibody PTK2B protein tyrosine kinase 2 beta antibody PYK2 antibody RAFTK antibody RAFTK2 antibody Related adhesion focal tyrosine kinase antibody |
| Product description | Focal adhesion kinase (FAK) was initially identified as a substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique compared to other protein families described. A putative new member of the FAK family, designated PYK2 (proline-rich tyrosine kinase 2), exhibits 61% sequence identity with FAK over its kinase domain. PYK2 (also designated CAKb or RAFTK) is highly expressed in the central nervous system. Activation of the kinase leads to modulation of ion channel function and the activation of the MAPK signaling pathway. PYK2 is rapidly phosphorylated on tyrosine residues in response to stimuli that increase intracellular calcium levels and compounds that activate members of the PKC family of kinases, such as phorbol esters. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CADTK antibody CAK-beta antibody CAKB antibody CAKbeta antibody Calcium regulated non receptor proline rich tyrosine kinase antibody Calcium-dependent tyrosine kinase antibody Cell adhesion kinase beta antibody E430023O05Rik antibody EC 2.7.10.2 antibody FADK 2 antibody FADK2 antibody FAK2 antibody FAK2_HUMAN antibody Focal adhesion kinase 2 antibody MGC124628 antibody PKB antibody Proline-rich tyrosine kinase 2 antibody Protein kinase B antibody Protein Tyrosine Kinase 2 Beta antibody Protein-tyrosine kinase 2-beta antibody PTK antibody PTK2B antibody PTK2B protein tyrosine kinase 2 beta antibody PYK2 antibody RAFTK antibody RAFTK2 antibody Related adhesion focal tyrosine kinase antibody |
|---|---|
| Molecular Weight(MW) | 116 kDa |
| Cellular Localization | Cytoplasm, Cell membrane, Cell junction, Cell projection, Nucleus. |
Application
Fig1: Western blot analysis of PYK2 on mouse brain lysates using anti-PYK2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining PYK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PYK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PYK2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PYK2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PYK2 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.| Positive Control | SHG-44, SH-SY-5Y, Hela, human kidney tissue, human tonsil tissue, mouse brain tissue, human spleen tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
