Application
Fig1: Western blot analysis of CD14 on different lysates using anti-CD14 antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver Lane 2: SW480Application
Fig2: ICC staining CD14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD14 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CD14 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining CD14 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD14 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD14 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD14 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD14 antibody. Counter stained with hematoxylin.Application
Fig10: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD14 antibody. Counter stained with hematoxylin.| Product Name | CD14 [SC69-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 14 antibody CD_antigen=CD14 antibody CD14 antibody CD14 antigen antibody CD14 molecule antibody CD14_HUMAN antibody LPS-R antibody Mo2 antibody Monocyte differentiation antigen CD14 antibody Monocyte differentiation antigen CD14 urinary form antibody Monocyte differentiation antigen CD14, membrane-bound form antibody Myeloid cell specific leucine rich glycoprotein antibody Myeloid cell-specific leucine-rich glycoprotein antibody |
| Product description | Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD 14 antibody
CD_antigen=CD14 antibody
CD14 antibody
CD14 antigen antibody
CD14 molecule antibody
CD14_HUMAN antibody
LPS-R antibody
Mo2 antibody
Monocyte differentiation antigen CD14 antibody
Monocyte differentiation antigen CD14 urinary form antibody
Monocyte differentiation antigen CD14 membrane-bound form antibody Myeloid cell specific leucine rich glycoprotein antibody Myeloid cell-specific leucine-rich glycoprotein antibody |
|---|---|
| Molecular Weight(MW) | 55-65 kDa |
| Cellular Localization | Cell membrane, Secreted, Membrane raft, Golgi apparatus. |
| SwissProt ID | P08571 |
|---|
Application
Fig1: Western blot analysis of CD14 on different lysates using anti-CD14 antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver Lane 2: SW480
Application
Fig2: ICC staining CD14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD14 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CD14 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining CD14 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD14 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD14 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD14 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD14 antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD14 antibody. Counter stained with hematoxylin.| Positive Control | A549, NCCIT, NIH/3T3, LO2, SW480, human colon cancer tissue, human spleen tissue, human tonsil tissue, human liver tissue, human uterus tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
