Application
Fig1: Western blot analysis of RALBP1 on different lysates using anti-RALBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HCT116 Lane 2: Jurkat Lane 3: 293TApplication
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of 293 cells with RALBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | RALBP1 [SC06-49] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RLIP1 antibody 76 kDa Ral-interacting protein antibody 76-kDa Ral-interacting protein antibody Dinitrophenyl S-glutathione ATPase antibody DNP-SG ATPase antibody Ral-interacting protein 1 antibody Ral-interacting protein 1, 76-KD antibody RalA-binding protein 1 antibody RalBP1 antibody RBP1_HUMAN antibody RIP1 antibody RLIP1 antibody RLIP76 antibody |
| Product description | Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells. A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain. This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42. A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | RLIP1 antibody
76 kDa Ral-interacting protein antibody
76-kDa Ral-interacting protein antibody
Dinitrophenyl S-glutathione ATPase antibody
DNP-SG ATPase antibody
Ral-interacting protein 1 antibody
Ral-interacting protein 1 76-KD antibody RalA-binding protein 1 antibody RalBP1 antibody RBP1_HUMAN antibody RIP1 antibody RLIP1 antibody RLIP76 antibody |
|---|---|
| Molecular Weight(MW) | 95 kDa |
| Cellular Localization | Membrane. |
Application
Fig1: Western blot analysis of RALBP1 on different lysates using anti-RALBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HCT116 Lane 2: Jurkat Lane 3: 293T
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of 293 cells with RALBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | HCT116, Jurkat, human breast carcinoma tissue, human pancreas tissue, mouse lung tissue, mouse pancreas tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
