Application
Fig1: Western blot analysis of IRF7 on different lysates using anti-IRF7 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: RajiApplication
Fig2: ICC staining IRF7 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IRF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining IRF7 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRF7 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IRF7 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IRF7 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-IRF7 antibody. Counter stained with hematoxylin.Application
Fig11: Flow cytometric analysis of Jurkat cells with IRF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibodyApplication
Fig12:Western blot analysis of IRF7 on hybrid fish (crucian-carp) brain tissue lysate using anti-IRF7 antibody at 1/500 dilution.| Product Name | IRF7 [SC0617] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IMD39 antibody Interferon regulatory factor 7 antibody Interferon regulatory factor 7H antibody IRF 7 antibody IRF 7A antibody IRF 7H antibody IRF-7 antibody IRF7 antibody IRF7_HUMAN antibody IRF7A antibody IRF7B antibody IRF7C antibody IRF7H antibody |
| Product description | Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-α and β) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-α and interferon-β genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7 and IFN consensus sequence-binding protein (ICSBP). |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | IMD39 antibody Interferon regulatory factor 7 antibody Interferon regulatory factor 7H antibody IRF 7 antibody IRF 7A antibody IRF 7H antibody IRF-7 antibody IRF7 antibody IRF7_HUMAN antibody IRF7A antibody IRF7B antibody IRF7C antibody IRF7H antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
Application
Fig1: Western blot analysis of IRF7 on different lysates using anti-IRF7 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Raji
Application
Fig2: ICC staining IRF7 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IRF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IRF7 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Application
Fig11: Flow cytometric analysis of Jurkat cells with IRF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Application
Fig12:Western blot analysis of IRF7 on hybrid fish (crucian-carp) brain tissue lysate using anti-IRF7 antibody at 1/500 dilution.| Positive Control | Jurkat, Raji, PC-12, HepG2, mouse liver tissue, human tonsil tissue, mouse kidney tissue, human spleen tissue, mouse heart tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
