Application
Fig1: Western blot analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12Application
Fig2: ICC staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | C/EBP Beta [SC55-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGP/EBP antibody C EBP beta antibody C/EBP beta antibody C/EBP related protein 2 antibody CCAAT Enhancer Binding Protein beta antibody CCAAT/enhancer-binding protein beta antibody CEBPB antibody CEBPB_HUMAN antibody CRP2 antibody IL 6DBP antibody IL6DBP antibody Interleukin 6 dependent binding protein antibody LAP antibody Liver activator protein antibody Liver enriched transcriptional activator antibody NF IL6 antibody NFIL6 antibody Nuclear factor NF IL6 antibody Nuclear factor NF-IL6 antibody SF B antibody SFB antibody Silencer factor B antibody TCF-5 antibody TCF5 antibody Transcription factor 5 antibody |
| Product description | CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP β is a member of the C/EBP transcription factor family. The C/EBP β gene encodes several isoforms containing truncated transcription activation domains due to the alternative translational initiation at multipe AUG start sites. C/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein (LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP β gene that has direct implication for acute phase response in liver cells.The C/EBP β form requires phosphorylation for its DNA binding ability, and increase binding of C/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AGP/EBP antibody C EBP beta antibody C/EBP beta antibody C/EBP related protein 2 antibody CCAAT Enhancer Binding Protein beta antibody CCAAT/enhancer-binding protein beta antibody CEBPB antibody CEBPB_HUMAN antibody CRP2 antibody IL 6DBP antibody IL6DBP antibody Interleukin 6 dependent binding protein antibody LAP antibody Liver activator protein antibody Liver enriched transcriptional activator antibody NF IL6 antibody NFIL6 antibody Nuclear factor NF IL6 antibody Nuclear factor NF-IL6 antibody SF B antibody SFB antibody Silencer factor B antibody TCF-5 antibody TCF5 antibody Transcription factor 5 antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12
Application
Fig2: ICC staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | PC-12, MCF-7, Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
