Application
Fig1: Western blot analysis of Hes1 on different lysates using anti-Hes1 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SH-SY-5YApplication
Fig2: ICC staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Hes1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of sh-sy-5y cells with Hes1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Hes1 [SC06-21] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bHLHb39 antibody C-HAIRY1 antibody c-hairy1A antibody Class B basic helix-loop-helix protein 39 antibody FLJ20408 antibody Hairy and enhancer of split 1 (Drosophila) antibody Hairy and enhancer of split 1 antibody Hairy homolog (Drosophila) antibody Hairy homolog antibody Hairy like antibody Hairy, Drosophila, homolog of antibody Hairy-like protein antibody Hairy/enhancer of split, Drosophila, homolog of, 1 antibody HAIRY1 antibody HES-1 antibody hes1 antibody Hes1 hairy and enhancer of split 1 (Drosophila) antibody HES1_HUMAN antibody HHL antibody HL antibody HRY antibody MGC129109 antibody OTTHUMP00000209031 antibody RHL antibody Transcription factor HES-1 antibody |
| Product description | The Drosophila Hairy and enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homolog of Drosophila Hairy and enhancer of split are the HES gene family members HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homolog of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | bHLHb39 antibody
C-HAIRY1 antibody
c-hairy1A antibody
Class B basic helix-loop-helix protein 39 antibody
FLJ20408 antibody
Hairy and enhancer of split 1 (Drosophila) antibody
Hairy and enhancer of split 1 antibody
Hairy homolog (Drosophila) antibody
Hairy homolog antibody
Hairy like antibody
Hairy Drosophila homolog of antibody Hairy-like protein antibody Hairy/enhancer of split Drosophila homolog of 1 antibody HAIRY1 antibody HES-1 antibody hes1 antibody Hes1 hairy and enhancer of split 1 (Drosophila) antibody HES1_HUMAN antibody HHL antibody HL antibody HRY antibody MGC129109 antibody OTTHUMP00000209031 antibody RHL antibody Transcription factor HES-1 antibody |
|---|---|
| Molecular Weight(MW) | 30 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of Hes1 on different lysates using anti-Hes1 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SH-SY-5Y
Application
Fig2: ICC staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Hes1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of sh-sy-5y cells with Hes1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | 293, MCF-7, Hela, SH-SY-5Y, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
