Application
Fig1: Western blot analysis of RhoA on rat brain lysates using anti-RhoA antibody at 1/1,000 dilution.Application
Fig2: ICC staining RhoA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of Hela cells with RhoA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | RhoA [SN0612] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aplysia ras related homolog 12 antibody ARH12 antibody ARHA antibody H 12 antibody H12 antibody Oncogene RHO H12 antibody Ras homolog family member A antibody Ras homolog gene family member A antibody Rho A antibody Rho cDNA clone 12 antibody RHO H12 antibody RHO12 antibody RHOA antibody RHOA_HUMAN antibody RHOH12 antibody Small GTP binding protein Rho A antibody Transforming protein Rho A antibody Transforming protein RhoA antibody |
| Product description | The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Aplysia ras related homolog 12 antibody ARH12 antibody ARHA antibody H 12 antibody H12 antibody Oncogene RHO H12 antibody Ras homolog family member A antibody Ras homolog gene family member A antibody Rho A antibody Rho cDNA clone 12 antibody RHO H12 antibody RHO12 antibody RHOA antibody RHOA_HUMAN antibody RHOH12 antibody Small GTP binding protein Rho A antibody Transforming protein Rho A antibody Transforming protein RhoA antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Cleavage furrow, Midbody, Cell projection. |
Application
Fig1: Western blot analysis of RhoA on rat brain lysates using anti-RhoA antibody at 1/1,000 dilution.
Application
Fig2: ICC staining RhoA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of Hela cells with RhoA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SH-SY-5Y, rat brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
