Application
Fig1: Western blot analysis of IRAKM on different lysates using anti-IRAKM antibody at 1/1,000 dilution. Positive control: Lane 1: Human placenta Lane 2: Human thyroid| Product Name | IRAKM [SN64-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASRT5 antibody IL 1 receptor associated kinase M antibody IL-1 receptor-associated kinase M antibody IL1 receptor associated kinase M antibody Interleukin 1 receptor associated kinase 3 antibody Interleukin 1 receptor associated kinase M antibody Interleukin-1 receptor-associated kinase 3 antibody IRAK M antibody IRAK-3 antibody IRAK-M antibody Irak3 antibody IRAK3_HUMAN antibody |
| Product description | Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs. Binding of IL-1 to its cognate receptor results in the activation of the NFkB signaling pathway. An IL-1-dependent kinase termed IRAK-1 (for IL-1 receptor- associated kinase 1) coimmunoprecipitates with activated IL-1RI and is implicated as an upstream mediator of NFkB activation. A related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFkB. IRAK-2 is a proximal mediator of IL1, a component of the IL1R signaling complex, and is required for IL1R-induced NFkB activation. IRAK-4, like IRAK-1 and Pelle, has auto- and cross-phosphorylation kinase activity. IRAK-4 is strongly expressed in kidney and is also found in lung, testis, small intestine, breast, liver, and placenta. In contrast to the other IRAKs that are expressed in most cell types, IRAK-M is restricted to monocytic cells. IRAK-M mRNA transcripts are found predominantly in PBL and the monocytic cell lines U937 and THP-1. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIP |
| WB:1:1,000-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ASRT5 antibody IL 1 receptor associated kinase M antibody IL-1 receptor-associated kinase M antibody IL1 receptor associated kinase M antibody Interleukin 1 receptor associated kinase 3 antibody Interleukin 1 receptor associated kinase M antibody Interleukin-1 receptor-associated kinase 3 antibody IRAK M antibody IRAK-3 antibody IRAK-M antibody Irak3 antibody IRAK3_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q9Y616 |
|---|
Application
Fig1: Western blot analysis of IRAKM on different lysates using anti-IRAKM antibody at 1/1,000 dilution. Positive control: Lane 1: Human placenta Lane 2: Human thyroid| Positive Control | Human placenta tissue, human thyroid tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
