Application
Fig1: Western blot analysis of Tyrosine Hydroxylase on PC-12 cells lysates using anti-Tyrosine Hydroxylase antibody at 1/1,000 dilution.Application
Fig2: ICC staining Tyrosine Hydroxylase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Tyrosine Hydroxylase in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Tyrosine Hydroxylase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tyrosine Hydroxylase antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of N2A cells with Tyrosine Hydroxylase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody| Product Name | Tyrosine Hydroxylase [SN59-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dystonia 14 antibody DYT14 antibody DYT5b antibody EC 1.14.16.2 antibody OTTHUMP00000011225 antibody OTTHUMP00000011226 antibody ple antibody Protein Pale antibody TH antibody The antibody TY3H_HUMAN antibody TYH antibody Tyrosine 3 hydroxylase antibody Tyrosine 3 monooxygenase antibody Tyrosine 3-hydroxylase antibody Tyrosine 3-monooxygenase antibody Tyrosine hydroxylase antibody |
| Product description | The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson's disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Dystonia 14 antibody DYT14 antibody DYT5b antibody EC 1.14.16.2 antibody OTTHUMP00000011225 antibody OTTHUMP00000011226 antibody ple antibody Protein Pale antibody TH antibody The antibody TY3H_HUMAN antibody TYH antibody Tyrosine 3 hydroxylase antibody Tyrosine 3 monooxygenase antibody Tyrosine 3-hydroxylase antibody Tyrosine 3-monooxygenase antibody Tyrosine hydroxylase antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Tyrosine Hydroxylase on PC-12 cells lysates using anti-Tyrosine Hydroxylase antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Tyrosine Hydroxylase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Tyrosine Hydroxylase in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Tyrosine Hydroxylase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tyrosine Hydroxylase antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of N2A cells with Tyrosine Hydroxylase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody| Positive Control | PC-12, N2A, NIH/3T3, SH-SY-5Y, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
