Application
Fig1: Western blot analysis of MUC1 on Hela cells lysates using anti-MUC1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining MUC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MUC1 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | MUC1 [SN06-80] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADMCKD antibody ADMCKD1 antibody Breast carcinoma associated antigen DF3 antibody Breast carcinoma-associated antigen DF3 antibody CA 15-3 antibody CA15 3 antibody CA15 3 antigen antibody CA15.3 antibody Cancer antigen 15-3 antibody Carcinoma associated mucin antibody Carcinoma-associated mucin antibody CD 227 antibody CD227 antibody DF3 antigen antibody EMA antibody Episialin antibody H23 antigen antibody H23AG antibody KL 6 antibody KL-6 antibody KL6 antibody Krebs von den Lungen-6 antibody MAM 6 antibody MAM6 antibody MCD antibody MCKD antibody MCKD1 antibody Medullary cystic kidney disease 1 (autosomal dominant) antibody Medullary cystic kidney disease, autosomal dominant antibody MUC 1 antibody MUC-1 antibody MUC-1/SEC antibody MUC-1/X antibody MUC1 antibody MUC1-alpha antibody MUC1-beta antibody MUC1-CT antibody MUC1-NT antibody MUC1/ZD antibody MUC1_HUMAN antibody Mucin 1 antibody Mucin 1 transmembrane antibody Mucin 1, cell surface associated antibody Mucin-1 subunit beta antibody Peanut reactive urinary mucin antibody Peanut-reactive urinary mucin antibody PEM antibody PEMT antibody Polymorphic epithelial mucin antibody PUM antibody Tumor associated epithelial membrane antigen antibody Tumor associated epithelial mucin antibody Tumor associated mucin antibody Tumor-associated epithelial membrane antigen antibody Tumor-associated mucin antibody |
| Product description | The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major com-ponent of various mucus gels and is broadly expressed in normal and tumor cells. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ADMCKD antibody
ADMCKD1 antibody
Breast carcinoma associated antigen DF3 antibody
Breast carcinoma-associated antigen DF3 antibody
CA 15-3 antibody
CA15 3 antibody
CA15 3 antigen antibody
CA15.3 antibody
Cancer antigen 15-3 antibody
Carcinoma associated mucin antibody
Carcinoma-associated mucin antibody
CD 227 antibody
CD227 antibody
DF3 antigen antibody
EMA antibody
Episialin antibody
H23 antigen antibody
H23AG antibody
KL 6 antibody
KL-6 antibody
KL6 antibody
Krebs von den Lungen-6 antibody
MAM 6 antibody
MAM6 antibody
MCD antibody
MCKD antibody
MCKD1 antibody
Medullary cystic kidney disease 1 (autosomal dominant) antibody
Medullary cystic kidney disease autosomal dominant antibody MUC 1 antibody MUC-1 antibody MUC-1/SEC antibody MUC-1/X antibody MUC1 antibody MUC1-alpha antibody MUC1-beta antibody MUC1-CT antibody MUC1-NT antibody MUC1/ZD antibody MUC1_HUMAN antibody Mucin 1 antibody Mucin 1 transmembrane antibody Mucin 1 cell surface associated antibody Mucin-1 subunit beta antibody Peanut reactive urinary mucin antibody Peanut-reactive urinary mucin antibody PEM antibody PEMT antibody Polymorphic epithelial mucin antibody PUM antibody Tumor associated epithelial membrane antigen antibody Tumor associated epithelial mucin antibody Tumor associated mucin antibody Tumor-associated epithelial membrane antigen antibody Tumor-associated mucin antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Apical cell membrane, Secreted, Cell membrane, Cytoplasm, Nucleus. |
| SwissProt ID | P15941 |
|---|
Application
Fig1: Western blot analysis of MUC1 on Hela cells lysates using anti-MUC1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining MUC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MUC1 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | B16-F1, Hela, human breast tissue, human kidney tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
