Application
Fig1: ICC staining MSK1(phospho S376) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining MSK1(phospho S376) in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MSK1(phospho S376) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MSK1(phospho S376) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-MSK1(phospho S376) antibody. Counter stained with hematoxylin.| Product Name | MSK1(phospho S376) [SN63-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 90 kDa ribosomal protein S6 kinase 5 antibody EC 2.7.11.1 antibody KS6A5_HUMAN antibody MGC1911 antibody Mitogen and stress activated protein kinase 1 antibody MSPK1 antibody Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody Nuclear mitogen- and stress-activated protein kinase 1 antibody Ribosomal protein S6 kinase 90kD polypeptide 5 antibody Ribosomal protein S6 kinase 90kDa antibody Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody Ribosomal Protein S6 Kinase Alpha 5 antibody Ribosomal protein S6 kinase alpha-5 antibody RLPK antibody RPS6KA5 antibody RSK Like Protein Kinase antibody RSK-like protein kinase antibody RSKL antibody S6K alpha 5 antibody S6K-alpha-5 antibody |
| Product description | The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms. |
| Immunogen | recombinant protein |
| Modification | p-S376 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIP |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | 90 kDa ribosomal protein S6 kinase 5 antibody EC 2.7.11.1 antibody KS6A5_HUMAN antibody MGC1911 antibody Mitogen and stress activated protein kinase 1 antibody MSPK1 antibody Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody Nuclear mitogen- and stress-activated protein kinase 1 antibody Ribosomal protein S6 kinase 90kD polypeptide 5 antibody Ribosomal protein S6 kinase 90kDa antibody Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody Ribosomal Protein S6 Kinase Alpha 5 antibody Ribosomal protein S6 kinase alpha-5 antibody RLPK antibody RPS6KA5 antibody RSK Like Protein Kinase antibody RSK-like protein kinase antibody RSKL antibody S6K alpha 5 antibody S6K-alpha-5 antibody |
|---|---|
| Molecular Weight(MW) | 90/80 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
Application
Fig1: ICC staining MSK1(phospho S376) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining MSK1(phospho S376) in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MSK1(phospho S376) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MSK1(phospho S376) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-MSK1(phospho S376) antibody. Counter stained with hematoxylin.| Positive Control | LO2, PC-3M, Hela, 293, mouse bladder tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
