Application
Fig1: ICC staining phospho-c-Myc(T58) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining phospho-c-Myc(T58) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of MCF-7 cells with phospho-c-Myc(T58) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | Phospho-c-Myc(T58) [SN60-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Avian myelocytomatosis viral oncogene homolog antibody bHLHe39 antibody c Myc antibody Class E basic helix-loop-helix protein 39 antibody MRTL antibody Myc antibody Myc protein antibody Myc proto oncogene protein antibody Myc proto-oncogene protein antibody myc related translation/localization regulatory factor antibody MYC_HUMAN antibody Myc2 antibody MYCC antibody Niard antibody Nird antibody Proto-oncogene c-Myc antibody Transcription factor p64 antibody v myc avian myelocytomatosis viral oncogene homolog antibody v myc myelocytomatosis viral oncogene homolog antibody |
| Product description | c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3. |
| Immunogen | recombinant protein |
| Modification | p-T58 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:1,000 FC:1:50-1:100 ICC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Avian myelocytomatosis viral oncogene homolog antibody bHLHe39 antibody c Myc antibody Class E basic helix-loop-helix protein 39 antibody MRTL antibody Myc antibody Myc protein antibody Myc proto oncogene protein antibody Myc proto-oncogene protein antibody myc related translation/localization regulatory factor antibody MYC_HUMAN antibody Myc2 antibody MYCC antibody Niard antibody Nird antibody Proto-oncogene c-Myc antibody Transcription factor p64 antibody v myc avian myelocytomatosis viral oncogene homolog antibody v myc myelocytomatosis viral oncogene homolog antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P01106 |
|---|
Application
Fig1: ICC staining phospho-c-Myc(T58) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining phospho-c-Myc(T58) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of MCF-7 cells with phospho-c-Myc(T58) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | SKOV-3, Hela. |
|---|---|
| Application Notes | WB:1:1,000 FC:1:50-1:100 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
