Application
Fig1: Western blot analysis of ATG5 on different lysates using anti-ATG5 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Hela Lane 3: PC12Application
Fig2: ICC staining ATG5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ATG5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ATG5 antibody. Counter stained with hematoxylin.| Product Name | ATG5 [SN73-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APG 5 antibody APG 5L antibody APG5 antibody APG5 autophagy 5 like antibody APG5 like antibody APG5-like antibody APG5L antibody Apoptosis specific protein antibody Apoptosis-specific protein antibody ASP antibody ATG 5 antibody Atg5 antibody ATG5 autophagy related 5 homolog antibody ATG5_HUMAN antibody Autophagy protein 5 antibody Autophagy related 5 antibody hAPG5 antibody Homolog of S Cerevisiae autophagy 5 antibody OTTHUMP00000040507 antibody |
| Product description | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | APG 5 antibody APG 5L antibody APG5 antibody APG5 autophagy 5 like antibody APG5 like antibody APG5-like antibody APG5L antibody Apoptosis specific protein antibody Apoptosis-specific protein antibody ASP antibody ATG 5 antibody Atg5 antibody ATG5 autophagy related 5 homolog antibody ATG5_HUMAN antibody Autophagy protein 5 antibody Autophagy related 5 antibody hAPG5 antibody Homolog of S Cerevisiae autophagy 5 antibody OTTHUMP00000040507 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Cytoplasm, Preautophagosomal structure membrane. |
Application
Fig1: Western blot analysis of ATG5 on different lysates using anti-ATG5 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Hela Lane 3: PC12
Application
Fig2: ICC staining ATG5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ATG5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ATG5 antibody. Counter stained with hematoxylin.| Positive Control | Raji, MCF-7, Hela, PC12, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
