Application
Fig1: Western blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: 293TApplication
Fig2: Flow cytometric analysis of Hela cells with NSD3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | NSD3 [SN07-11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp667H044 antibody FLJ20353 antibody Histone lysine N methyltransferase NSD3 antibody Histone-lysine N-methyltransferase NSD3 antibody MGC126766 antibody MGC142029 antibody NSD 3 antibody NSD3_HUMAN antibody Nuclear set domain containing 3 antibody Nuclear SET domain containing protein 3 antibody Nuclear SET domain-containing protein 3 antibody pp14328 antibody Protein whistle antibody Whistle antibody WHSC1 like protein 1 antibody WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody WHSC1-like protein 1 antibody WHSC1L1 antibody WHSC1L1 protein antibody Wolf Hirschhorn syndrome candidate 1 like 1 antibody Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody |
| Product description | The deduced 1,437 amino acid NSD3 protein contains two PWWP domains involved in protein-protein interactions, five PHD-type zinc finger motifs found in chromatin-associated proteins, a SAC (SET-associated cys-rich) domain, a SET domain and a C-terminal C5HCH domain. Two NSD3 variants have been identified. The short variant comprised of 645 amino acids, arises from alternative polyadenylation and exon splicing and contains a single PWWP domain. A longer NSD3 variant, which is only expressed in HeLa cells, is comprised of 1,388 amino acid residues. The human WHSC1L1 gene, which encodes the NSD3 protein, shares 68% and 55% identity with mouse Nsd1 and human WHSC1, respectively. Highest expression of NSD3 is observed in brain, heart and skeletal muscle tissues; lower levels of NSD3 expression are observed in the liver and lungs. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp667H044 antibody FLJ20353 antibody Histone lysine N methyltransferase NSD3 antibody Histone-lysine N-methyltransferase NSD3 antibody MGC126766 antibody MGC142029 antibody NSD 3 antibody NSD3_HUMAN antibody Nuclear set domain containing 3 antibody Nuclear SET domain containing protein 3 antibody Nuclear SET domain-containing protein 3 antibody pp14328 antibody Protein whistle antibody Whistle antibody WHSC1 like protein 1 antibody WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody WHSC1-like protein 1 antibody WHSC1L1 antibody WHSC1L1 protein antibody Wolf Hirschhorn syndrome candidate 1 like 1 antibody Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 162 kDa |
| Cellular Localization | Nucleus, Chromosome. |
Application
Fig1: Western blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: 293T
Application
Fig2: Flow cytometric analysis of Hela cells with NSD3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | MCF-7, 293T. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
