WB
Western blot analysis of GST on different lysates with Rabbit anti-GST antibody at 1/50,000 dilution. Lane 1: 293T transfected with GST cell lysate (2.5 µg/Lane), Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane), Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa transfected with GST-tagged Histone H3.1 cells labeling GST with Rabbit anti-GST antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Histone H3 (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IP
GST was immunoprecipitated in 0.2mg 293T-OE-GST cell lysate with Rabbit anti-GST antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-GST antibody at 1/50,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate (input), Lane 2: Rabbit IgG instead of Rabbit anti-GST antibody in 293T transfected with GST-tagged Histone H3.1 cell lysate, Lane 3: Rabbit anti-GST antibody IP in 293T transfected with GST-tagged Histone H3.1 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 24 seconds.| Product Name | GST Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest. |
| Immunogen | GST tag recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:1000-1:50000 ICC/IF:1:50-1:200 IP:Use at an assay dependent concentration. |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Glutathione S Transferase antibody Glutathione S-transferase class-mu 26 kDa isozyme antibody GST antibody Sj26 antigen antibody SjGST antibody |
|---|
WB
Western blot analysis of GST on different lysates with Rabbit anti-GST antibody at 1/50,000 dilution. Lane 1: 293T transfected with GST cell lysate (2.5 µg/Lane), Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane), Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa transfected with GST-tagged Histone H3.1 cells labeling GST with Rabbit anti-GST antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Histone H3 (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IP
GST was immunoprecipitated in 0.2mg 293T-OE-GST cell lysate with Rabbit anti-GST antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-GST antibody at 1/50,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate (input), Lane 2: Rabbit IgG instead of Rabbit anti-GST antibody in 293T transfected with GST-tagged Histone H3.1 cell lysate, Lane 3: Rabbit anti-GST antibody IP in 293T transfected with GST-tagged Histone H3.1 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 24 seconds.| Positive Control | GST tag recombinant protein |
|---|---|
| Application Notes | WB:1:1000-1:50000 ICC/IF:1:50-1:200 IP:Use at an assay dependent concentration. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
