Application
Fig1: Western blot analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293Application
Fig2: ICC staining Glutaminase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Glutaminase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.| Product Name | Glutaminase [SN68-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AAD20 antibody DKFZp686O15119 antibody FLJ10358 antibody GAC antibody GAM antibody GLS antibody GLS1 antibody GLSK_HUMAN antibody Glutaminase C antibody Glutaminase kidney isoform antibody Glutaminase phosphate activated antibody K-glutaminase antibody KGA antibody KIAA0838 antibody L-glutamine amidohydrolase antibody mitochondrial antibody |
| Product description | Glutamine is an important molecule involved in several cellular functions, including nitrogen and carbon transport, hepatic urea synthesis, renal ammoniagenesis, and gluconeogenesis. Glutamine is catabolized by either the liver-type (LGA) or kidney-type (KGA) glutaminase. KGA is mitochondrial specific protein whose expression in kidney is increased during metabolic acidosis. This process is mediated by an 8-base AU-sequence in KGA that functions as a pH-response element. The human KGA gene maps to chromosome 2, and produces three isoforms, designated KGA, GAC, and GAM, by alternative splicing. KGA is synthesized as a cytosolic protein that is transported to the mitochondria as an intermediate protein, and is further cleaved into the KGA isoform and the GAC isoform. The processing of the GAM isoform is unclear. The KGA isoform is abundant in brain and kidney, while the GAC isoform is principally expressed in cardiac muscle and pancreas. The GAM isoform is solely expressed in cardiac and skeletal muscle. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AAD20 antibody DKFZp686O15119 antibody FLJ10358 antibody GAC antibody GAM antibody GLS antibody GLS1 antibody GLSK_HUMAN antibody Glutaminase C antibody Glutaminase kidney isoform antibody Glutaminase phosphate activated antibody K-glutaminase antibody KGA antibody KIAA0838 antibody L-glutamine amidohydrolase antibody mitochondrial antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Cytoplasm, Mitochondrion. |
Application
Fig1: Western blot analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293
Application
Fig2: ICC staining Glutaminase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Glutaminase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.| Positive Control | 293, Hela, HepG2, human tonsil tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
