Application
Fig1: Western blot analysis of Phospho-Cdk2(Y15) on different lysates using anti-Phospho-Cdk2(Y15) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen Lane 3: NIH/3T3Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.| Product Name | Phospho-Cdk2(Y15) [SN72-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cdc2 related protein kinase antibody cdc2-related protein kinase antibody CDC28 antibody CDC2A antibody Cdk 2 antibody CDK1 antibody CDK2 antibody CDK2_HUMAN antibody CDKN2 antibody Cell devision kinase 2 antibody Cell division protein kinase 2 antibody Cyclin dependent kinase 2 antibody cyclin dependent kinase 2-alpha antibody Cyclin-dependent kinase 2 antibody kinase Cdc2 antibody MPF antibody p33 protein kinase antibody p33(CDK2) antibody |
| Product description | In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr15 of human Cdk2. |
| Modification | p-Y15 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:1,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cdc2 related protein kinase antibody cdc2-related protein kinase antibody CDC28 antibody CDC2A antibody Cdk 2 antibody CDK1 antibody CDK2 antibody CDK2_HUMAN antibody CDKN2 antibody Cell devision kinase 2 antibody Cell division protein kinase 2 antibody Cyclin dependent kinase 2 antibody cyclin dependent kinase 2-alpha antibody Cyclin-dependent kinase 2 antibody kinase Cdc2 antibody MPF antibody p33 protein kinase antibody p33(CDK2) antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Endosome. |
Application
Fig1: Western blot analysis of Phospho-Cdk2(Y15) on different lysates using anti-Phospho-Cdk2(Y15) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen Lane 3: NIH/3T3
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, Hela, human breast carcinoma tissue, mouse spleen tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
