Application
Fig1: ICC staining Cytokeratin 4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Cytokeratin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cytokeratin 4 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 4 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of A431 cells with Cytokeratin 4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Cytokeratin 4 [SN74-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CK 4 antibody CK-4 antibody CK4 antibody CYK4 antibody Cytokeratin 4 antibody Cytokeratin-4 antibody Cytokeratin4 antibody FLJ31692 antibody K2C4_HUMAN antibody K4 antibody Keratin 4 antibody Keratin antibody Keratin type II cytoskeletal 4 antibody Keratin-4 antibody Keratin4 antibody KRT 4 antibody Krt4 antibody type II cytoskeletal 4 antibody Type-II keratin Kb4 antibody |
| Product description | Cytokeratins are a subfamily of intermediate filament keratins that are characterized by a remarkable biochemical diversity, which is represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in isoelectric range between 4.9 and 7.8. Cytokeratin 1 has the highest molecular weight, while Cytokeratin 19 has the lowest molecular weight. The cytokeratins are divided into the type I and type II subgroups. Type II family members comprise the basic to neutral members, Cytokeratins 1-8, while the type I group comprises the acidic members, Cytokeratins 9-20. Various epithelia in the human body usually express cytokeratins which are characteristic of the type of epithelium and related to the degree of maturation or differentiation within said epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. Cytokeratin 4 is expressed in differentiated layers of the mucosal and esophageal epithelia along with Cytokeratin 13. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CK 4 antibody CK-4 antibody CK4 antibody CYK4 antibody Cytokeratin 4 antibody Cytokeratin-4 antibody Cytokeratin4 antibody FLJ31692 antibody K2C4_HUMAN antibody K4 antibody Keratin 4 antibody Keratin antibody Keratin type II cytoskeletal 4 antibody Keratin-4 antibody Keratin4 antibody KRT 4 antibody Krt4 antibody type II cytoskeletal 4 antibody Type-II keratin Kb4 antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Cell surface, intermediate filament cytoskeleton, nucleus. |
| SwissProt ID | P19013 |
|---|
Application
Fig1: ICC staining Cytokeratin 4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Cytokeratin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cytokeratin 4 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 4 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of A431 cells with Cytokeratin 4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, SW480, Hela, human tonsil tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
