Application
Fig1: ICC staining CD10 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining CD10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD10 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD10 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Jurkat cells with CD10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | CD10 [SN75-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Atriopeptidase antibody CALLA antibody CD10 antibody CD10 antigen antibody Common acute lymphocytic leukemia antigen antibody DKFZp686O16152 antibody EC 3.4.24.11 antibody Enkephalinase antibody EPN antibody Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody Membrane metallo endopeptidase antibody Membrane metallo endopeptidase variant 1 antibody Membrane metallo endopeptidase variant 2 antibody Membrane metalloendopeptidase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody Membrane metalloendopeptidase variant 1 antibody Membrane metalloendopeptidase variant 2 antibody MGC126681 antibody MGC126707 antibody MME antibody NEP antibody NEP_HUMAN antibody Neprilysin antibody neprilysin-390 antibody neprilysin-411 antibody Neutral endopeptidase 24.11 antibody Neutral endopeptidase antibody Neutral endopeptidase, membrane-associated antibody SFE antibody Skin fibroblast elastase antibody |
| Product description | CD10, also called the common acute lymphoblastic leukemia antigen (CALLA) and neutral endopeptidase (NEP), is a type II integral membrane glycoprotein. CD10 acts as a zinc metalloprotease that cleaves a variety of biologically active peptides including Angiotensins I and II. It is expressed on early B and T lymphoid precursors, B blasts, some granulocytes, bone marrow stromal cells and certain epithelial cells including some tumor cell lines. CD10 is used as a marker of common acute lymphocytic leukemias and some lymphomas. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFCIHCICC |
| WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:50-1:100 IHC:1:50-1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Atriopeptidase antibody
CALLA antibody
CD10 antibody
CD10 antigen antibody
Common acute lymphocytic leukemia antigen antibody
DKFZp686O16152 antibody
EC 3.4.24.11 antibody
Enkephalinase antibody
EPN antibody
Membrane metallo endopeptidase (neutral endopeptidase enkephalinase) antibody Membrane metallo endopeptidase (neutral endopeptidase enkephalinase CALLA CD10) antibody Membrane metallo endopeptidase antibody Membrane metallo endopeptidase variant 1 antibody Membrane metallo endopeptidase variant 2 antibody Membrane metalloendopeptidase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody Membrane metalloendopeptidase variant 1 antibody Membrane metalloendopeptidase variant 2 antibody MGC126681 antibody MGC126707 antibody MME antibody NEP antibody NEP_HUMAN antibody Neprilysin antibody neprilysin-390 antibody neprilysin-411 antibody Neutral endopeptidase 24.11 antibody Neutral endopeptidase antibody Neutral endopeptidase membrane-associated antibody SFE antibody Skin fibroblast elastase antibody |
|---|---|
| Molecular Weight(MW) | 100 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: ICC staining CD10 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining CD10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Jurkat cells with CD10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | 293, MCF-7, human tonsil tissue, human breast carcinoma tissue, human kidney tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:50-1:100 IHC:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
