Application
Fig1: Western blot analysis of Calnexin on Hela cells lysates using anti-Calnexin antibody at 1/1,000 dilution.Application
Fig2: ICC staining Calnexin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Calnexin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Calnexin in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Calnexin antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calnexin antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.Application
Fig10: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.Application
Fig11: Flow cytometric analysis of Hela cells with Calnexin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | Calnexin [SN20-54] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Calnexin antibody CALX_HUMAN antibody CANX antibody CNX antibody FLJ26570 antibody Histocompatibility complex class I antigen binding protein p88 antibody IP90 antibody Major histocompatibility complex class I antigen-binding protein p88 antibody p90 antibody |
| Product description | Calnexin and Calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | Calnexin antibody CALX_HUMAN antibody CANX antibody CNX antibody FLJ26570 antibody Histocompatibility complex class I antigen binding protein p88 antibody IP90 antibody Major histocompatibility complex class I antigen-binding protein p88 antibody p90 antibody |
|---|---|
| Molecular Weight(MW) | 90 kDa |
| Cellular Localization | Endoplasmic reticulum membrane, Endoplasmic reticulum, Melanosome. |
Application
Fig1: Western blot analysis of Calnexin on Hela cells lysates using anti-Calnexin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Calnexin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Calnexin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Calnexin in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Application
Fig11: Flow cytometric analysis of Hela cells with Calnexin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | PANC-1, Hela, HepG2, rat kidney tissue, rat heart tissue, human kidney tissue, rat pancreas tissue, human pancreas tissue, human liver cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
