Application
Fig1: Western blot analysis of RAB7 on different lysates using anti-RAB7 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: C2C12Application
Fig2: ICC staining RAB7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining RAB7 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining RAB7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RAB7 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-RAB7 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of K562 cells with RAB7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | RAB7 [SN202-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMT2B antibody PRO2706 antibody PSN antibody RAB7, member RAS oncogene family antibody RAB7A antibody RAB7A, member RAS oncogene family antibody RAB7A_HUMAN antibody Ras associated protein RAB7 antibody Ras related protein Rab7 antibody Ras related protein Rab7a antibody Ras-related protein Rab-7a antibody |
| Product description | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CMT2B antibody
PRO2706 antibody
PSN antibody
RAB7 member RAS oncogene family antibody RAB7A antibody RAB7A member RAS oncogene family antibody RAB7A_HUMAN antibody Ras associated protein RAB7 antibody Ras related protein Rab7 antibody Ras related protein Rab7a antibody Ras-related protein Rab-7a antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Cytoplasmic vesicle, Lysosome membrane, Melanosome membrane, Lipid droplet, . |
Application
Fig1: Western blot analysis of RAB7 on different lysates using anti-RAB7 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: C2C12
Application
Fig2: ICC staining RAB7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining RAB7 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining RAB7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of K562 cells with RAB7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, C2C12, Hela, HepG2, SW480, mouse kidney tissue, human colon cancer tissue, human kidney tissue, mouse skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
