Application
Fig1: Western blot analysis of ARF1 on NIH/3T3 cells lysates using anti-ARF1 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ARF1 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Hela cells with ARF1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | ARF1 [SD2002] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP Ribosylation Factor 1 antibody ADP-ribosylation factor 1 antibody ARF 1 antibody ARF1 antibody ARF1_HUMAN antibody |
| Product description | ADP-ribosylation factors (ARFs), are small guanine nucleotide-binding proteins that enhance the enzymatic activities of cholera toxin, and constitute one family of the RAS superfamily. ARFs are essential and ubiquitous in eukaryotes, as they are involved in vesicular transport and functioning via phospholipase D activation. ARF proteins play a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. ARF1 is regulated by the binding and hydrolysis of GTP. Coatomer, or COPI, is a heptameric protein recruited to membranes by ARF1. Research demonstrates that guanine nucleotide exchange-activated ARF1, when located at the Golgi membrane, recruits and binds cytoplasmic COPI to the membranes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ADP Ribosylation Factor 1 antibody ADP-ribosylation factor 1 antibody ARF 1 antibody ARF1 antibody ARF1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Golgi apparatus, Cytoplasm, Cell junction , Membrane. |
Application
Fig1: Western blot analysis of ARF1 on NIH/3T3 cells lysates using anti-ARF1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ARF1 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Hela cells with ARF1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | NIH/3T3, human colon cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
