Application
Fig1: ICC staining S100A4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining S100A4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining S100A4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-S100A4 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100A4 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Jurkat cells with S100A4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | S100A4 [SD200-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 18A2 antibody 42A antibody calcium Placental protein antibody Calvasculin antibody CAPL antibody Fibroblast specific protein 1 (FSP1) antibody Fibroblast specific protein 1 antibody Fibroblast specific protein antibody FSP1 antibody Leukemia multidrug resistance associated protein antibody Malignant transformation suppression 1 (MTS1) antibody Malignant transformation suppression 1 antibody Metastasin antibody MTS1 antibody OTTHUMP00000015467 antibody OTTHUMP00000015468 antibody P9KA antibody PEL98 antibody Placental calcium-binding protein antibody Protein Mts1 antibody Protein S100 A4 antibody Protein S100-A4 antibody S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody S100 calcium binding protein A4 antibody S100 calcium-binding protein A4 antibody S100a4 antibody S10A4_HUMAN antibody |
| Product description | The Mts1 gene encodes a small acidic Ca2+-binding protein, Mts1 (also designated S100A4, calvasculin or metastasin). Mts1 belongs to the S100 family of small Ca2+-binding proteins and is expressed in a cell-specific manner. Mts1 protein is involved in tumor progression and metastasis, and also has a significant stimulatory effect on angiogenesis. The level of Mts1 protein in serum increases with aging, suggesting that Mts1 may play a role in the ind-uction of tumor progression via stimulation of angiogenesis. In addition, Mts1 cooperates with p53 in apoptosis induction by binding to the C-term-inal regulatory domain of p53 to inhibit the DNA binding activity of p53. The ability of Mts1 to enhance p53-dependent apoptosis may accelerate the loss of p53 function in tumors. Thus, Mts1 can contribute to the development of a more aggressive phenotype during tumor progression. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 18A2 antibody
42A antibody
calcium Placental protein antibody
Calvasculin antibody
CAPL antibody
Fibroblast specific protein 1 (FSP1) antibody
Fibroblast specific protein 1 antibody
Fibroblast specific protein antibody
FSP1 antibody
Leukemia multidrug resistance associated protein antibody
Malignant transformation suppression 1 (MTS1) antibody
Malignant transformation suppression 1 antibody
Metastasin antibody
MTS1 antibody
OTTHUMP00000015467 antibody
OTTHUMP00000015468 antibody
P9KA antibody
PEL98 antibody
Placental calcium-binding protein antibody
Protein Mts1 antibody
Protein S100 A4 antibody
Protein S100-A4 antibody
S100 calcium binding protein A4 (calcium protein calvasculin metastasin murine placental homolog) antibody S100 calcium binding protein A4 antibody S100 calcium-binding protein A4 antibody S100a4 antibody S10A4_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Extracellular space, nucleus |
Application
Fig1: ICC staining S100A4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining S100A4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining S100A4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-S100A4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100A4 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Jurkat cells with S100A4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | NIH/3T3, MCF-7, Hela, human tonsil tissue, mouse lung tissue, human lung tissue, human gastric carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
